Guerrero Luis, Herrera Emma, Salinas Juana, Torres Jesús, Montero Ana Bertha, Barrón Blanca
Laboratory of Virology, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México, Mexico.
Intervirology. 2008;51(4):285-92. doi: 10.1159/000170903. Epub 2008 Nov 6.
Infectious pancreatic necrosis virus (IPNV) is a birnavirus that causes a lethal disease in both hatchery-reared juvenile salmonids and other non-salmonid fishes. IPNV has been classified into seven genogroups based on the analysis of the VP2/NS junction region of the viral A RNA segment.
Ten organisms from two trout-rearing farms were used for viral isolation in RTG-2 cells. Cells were inoculated with samples from spleen, kidneys and pyloric caeca. The viral isolate was initially identified by electron microscopy, and confirmed by a commercial ELISA, RT-PCR and sequencing. A phylogenetic tree was constructed based on the deduced amino acids sequence of VP2.
An IPNV genogroup 1, labeled as EdoMex07, was isolated from a pool of renal tissues of five asymptomatic trout. The amino acid sequence analysis of VP2 showed that this IPNV presented the putative VP2 residue (221) already described in asymptomatic trout carriers.
The EdoMex07 IPNV isolate belongs to genogroup 1, and has a VP2 phenotype which has been suggested to be involved in the establishment of the carrier state. This EdoMex07 IPNV is currently used as the standard positive control for detection of IPNV in rainbow trout farms in Mexico.
传染性胰腺坏死病毒（IPNV）是一种双RNA病毒，可在孵化场养殖的幼年鲑科鱼类和其他非鲑科鱼类中引发致命疾病。基于对病毒A RNA片段的VP2/NS连接区的分析，IPNV已被分为七个基因组。
从两个鳟鱼养殖场采集了10个生物体样本，用于在RTG-2细胞中进行病毒分离。用脾脏、肾脏和幽门盲囊的样本接种细胞。最初通过电子显微镜鉴定病毒分离株，并通过商业ELISA、RT-PCR和测序进行确认。基于推导的VP2氨基酸序列构建了系统发育树。
从5条无症状鳟鱼的肾脏组织样本中分离出一株IPNV基因组1型毒株，标记为EdoMex07。VP2的氨基酸序列分析表明，该IPNV呈现出无症状鳟鱼携带者中已描述的假定VP2残基（221）。
EdoMex07 IPNV分离株属于基因组1型，具有一种VP2表型，据推测该表型与携带状态的建立有关。目前，这种EdoMex07 IPNV被用作墨西哥虹鳟鱼养殖场中IPNV检测的标准阳性对照。
