UXT是一种对细胞生存能力至关重要的新型中心体蛋白。
UXT is a novel centrosomal protein essential for cell viability.
作者信息
Zhao Huiwu, Wang Qiang, Zhang Hongtao, Liu Qingdu, Du Xiulian, Richter Mark, Greene Mark I
机构信息
Department of Pathology, University of Pennsylvania, Philadelphia, PA 19104, USA.
出版信息
Mol Biol Cell. 2005 Dec;16(12):5857-65. doi: 10.1091/mbc.e05-08-0705. Epub 2005 Oct 12.
Abstract
Ubiquitously expressed transcript (UXT) is a prefoldinlike protein that has been suggested to be involved in human tumorigenesis. Here, we have found that UXT is overexpressed in a number of human tumor tissues but not in the matching normal tissues. We demonstrate that UXT is located in human centrosomes and is associated with gamma-tubulin. In addition, overexpression of UXT disrupts centrosome structure. Furthermore, abrogation of UXT protein expression by small interfering RNA knockdown leads to cell death. Together, our findings suggest that UXT is a component of centrosome and is essential for cell viability. We propose that UXT may facilitate transformation by corrupting regulated centrosome functions.
摘要
泛表达转录本（UXT）是一种类似前折叠素的蛋白质，有人认为它参与了人类肿瘤的发生。在此，我们发现UXT在多种人类肿瘤组织中过表达，而在匹配的正常组织中则无此现象。我们证明UXT定位于人类中心体并与γ-微管蛋白相关。此外，UXT的过表达会破坏中心体结构。此外，通过小干扰RNA敲低消除UXT蛋白表达会导致细胞死亡。总之，我们的研究结果表明UXT是中心体的一个组成部分，对细胞活力至关重要。我们提出，UXT可能通过破坏受调控的中心体功能来促进细胞转化。
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