Thorsen B K, Enger O, Norland S, Hoff K A
Department of Microbiology and Plant Physiology, University of Bergen, Norway.
Appl Environ Microbiol. 1992 May;58(5):1624-8. doi: 10.1128/aem.58.5.1624-1628.1992.
Cultures of three strains of the fish pathogenic bacterium Yersinia ruckeri survived starvation in unsupplemented water for at least 4 months. At salinities of 0 to 20/1000 there were no detectable changes in CFU during the first 3 days of starvation and only a small decrease during the following 4 months, whereas at 35/1000 salinity, the survival potential of the cultures was markedly reduced. These results suggest that Y. ruckeri may survive for long periods in freshwater and brackish environments after an outbreak of enteric redmouth disease. Survival was also examined by use of the direct viable count method, and we show that this method can be combined with flow cytometry for automatic counting of viable bacteria. By flow cytometry, it was shown that genome replication initiated before the onset of starvation was completed, during the initial phase of starvation, and that starved cells could contain up to six genomes per cell.
三种鱼类致病性鲁氏耶尔森菌菌株在未添加营养物质的水中饥饿存活至少4个月。在盐度为0至20‰时，饥饿的前3天CFU没有可检测到的变化，在接下来的4个月中仅有少量减少，而在盐度为35‰时，培养物的存活潜力显著降低。这些结果表明，在肠道红嘴病爆发后，鲁氏耶尔森菌可能在淡水和微咸水环境中长期存活。还使用直接活菌计数法检测了存活率，并且我们表明该方法可以与流式细胞术结合用于活菌的自动计数。通过流式细胞术表明，在饥饿开始前基因组复制就已启动，在饥饿的初始阶段，饥饿细胞每个细胞可能含有多达六个基因组。
