Moraes Maria C B, Millar Jocelyn G, Laumann Raul A, Sujii Edison R, Pires Carmen S S, Borges Miguel
Embrapa Recursos Genéticos e Biotecnología, 02372, CEP 70849-970, Brasília, DF, Brazil.
J Chem Ecol. 2005 Jun;31(6):1415-27. doi: 10.1007/s10886-005-5294-1.
Olfactometer bioassays showed that odors from mature Thyanta perditor males attracted females but not males. Furthermore, odors from females did not attract either sex, indicating that like other phytophagous pentatomid bugs, the males produce a sex pheromone. Attraction appeared to peak in late afternoon to evening. The headspace volatiles collected from male and female T. perditor were analyzed by GC-MS and HPLC. A male-specific compound, methyl (2E,4Z,6Z)-decatrienoate (2E,4Z,6Z-10:COOMe), was identified along with a number of other compounds found in extracts from both sexes. Bioassays carried out with 2E,4Z,6Z-10:COOMe showed it was as attractive to females as the crude extract of male volatiles, suggesting that the male-produced sex pheromone consists of 2E,4Z,6Z-10:COOMe as a single component. Consecutive volatiles collections from males showed that 2E,4Z,6Z-10:COOMe began appearing in extracts from males about 9 d after the final molt, as the males became sexually mature.
嗅觉测定生物测定表明，成年珀蝽雄虫释放的气味能吸引雌虫，但不能吸引雄虫。此外，雌虫释放的气味对两性均无吸引力，这表明与其他植食性蝽象一样，雄虫会产生性信息素。吸引力在傍晚时分似乎达到峰值。利用气相色谱-质谱联用仪（GC-MS）和高效液相色谱仪（HPLC）对珀蝽雌雄虫的顶空挥发物进行了分析。除了在两性提取物中均发现的一些其他化合物外，还鉴定出一种雄性特异性化合物，即（2E,4Z,6Z）-癸三烯酸甲酯（2E,4Z,6Z-10:COOMe）。用2E,4Z,6Z-10:COOMe进行的生物测定表明，它对雌虫的吸引力与雄虫挥发物的粗提物相同，这表明雄虫产生的性信息素由单一成分2E,4Z,6Z-10:COOMe组成。对雄虫连续收集挥发物表明，随着雄虫性成熟，2E,4Z,6Z-10:COOMe在最后一次蜕皮后约9天开始出现在雄虫提取物中。
