Zhou Hongchun, Du Jiawei, Huang Yongping
Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, The Chinese Academy of Sciences, China.
J Chem Ecol. 2005 Jul;31(7):1645-56. doi: 10.1007/s10886-005-5804-1.
We evaluated the sublethal effects of malathion treatment on the behavioral responses of male Asian corn borers, Ostrinia furnacalis (Guenée), to their sex pheromones. Doses of malathion causing 1-50% mortality were topically applied to Asian corn borer male moths. The survivors were tested for behavioral and electroantennogram (EAG) responses to their sex pheromones. Sublethal doses of malathion affected males' ability to locate a pheromone source. In wind tunnel tests, male moths treated with sublethal doses of malathion took 2.5 to 12 times as long to take flight as control moths. Malathion-treated male moths were 60-96% less likely to locate a sex pheromone source than control moths. Multiple linear regression analysis revealed that the proportions of males performing each key stage were significantly correlated with the doses of malathion, but not with the time since treatment. Sensitivity and specificity of perception to pheromones in male moths were also disrupted by sublethal doses of malathion. The EAG responses of males treated with malathion decreased 0.13 mV on average compared with control moths. In addition, the ratios of pheromone components that elicited the largest responses shifted from approximately 5:5 to 1:9 (E/Z) and 9:1. We conclude that treatment with sublethal doses of malathion significantly reduced the likelihood that male Asian corn borers could successfully locate a normal female releasing sex pheromones.
我们评估了马拉硫磷处理对雄性亚洲玉米螟（Ostrinia furnacalis (Guenée)）对其性信息素行为反应的亚致死效应。将导致1 - 50%死亡率的马拉硫磷剂量局部施用于亚洲玉米螟雄蛾。对存活者进行了对其性信息素的行为和触角电位（EAG）反应测试。亚致死剂量的马拉硫磷影响了雄蛾定位性信息素源的能力。在风洞试验中，用亚致死剂量马拉硫磷处理的雄蛾起飞所需时间是对照雄蛾的2.5至12倍。经马拉硫磷处理的雄蛾定位性信息素源的可能性比对照雄蛾低60 - 96%。多元线性回归分析表明，处于每个关键阶段的雄蛾比例与马拉硫磷剂量显著相关，但与处理后的时间无关。亚致死剂量的马拉硫磷也破坏了雄蛾对性信息素感知的敏感性和特异性。与对照雄蛾相比，经马拉硫磷处理的雄蛾的EAG反应平均降低了0.13 mV。此外，引起最大反应的性信息素成分比例从大约5:5变为1:9（E/Z）和9:1。我们得出结论，用亚致死剂量的马拉硫磷处理显著降低了雄性亚洲玉米螟成功定位释放性信息素的正常雌蛾的可能性。
