一种通过毛细管气相色谱法定量赞比亚巴豆叶中松脂烷和羽扇豆烷二萜类化合物的有效方法。

A validated method for the quantification of pimarane and trachylobane diterpenes in the leaves of Croton zambesicus by capillary gas chromatography.

作者信息

Block Sébastien, Brkic Dejan, Hubert Philippe, Quetin-Leclercq Joëlle

机构信息

Laboratoire de Pharmacognosie, Unité d'Analyse Chimique et Physico-Chimique des Médicaments, Université Catholique de Louvain, Bruxelles, Belgium.

出版信息

Phytochem Anal. 2005 Sep-Oct;16(5):342-8. doi: 10.1002/pca.855.

DOI:10.1002/pca.855

PMID:16223091

Abstract

A sensitive and accurate method, combining Soxhlet extraction, solid-phase extraction and capillary gas chromatography, is described for the quantitative determination of four new diterpenes (ent-trachyloban-3beta-ol, ent-18-hydroxy-trachyloban-3-one, ent-trachyloban-3-one and isopimara-7,15-dien-3beta-ol) from the leaves of Croton zambesicus. This is the first method describing the quantification of trachylobane diterpenes in a crude extract. It has been fully validated in order to be able to compare the diterpene composition in other samples of C. zambesicus, which is an important source of trachylobanes.

摘要

描述了一种灵敏且准确的方法，该方法结合索氏提取、固相萃取和毛细管气相色谱法，用于定量测定赞比亚巴豆叶中的四种新二萜类化合物（对映-曲洛班-3β-醇、对映-18-羟基-曲洛班-3-酮、对映-曲洛班-3-酮和异海松-7,15-二烯-3β-醇）。这是第一种描述粗提物中曲洛班二萜类化合物定量的方法。该方法已得到充分验证，以便能够比较赞比亚巴豆其他样品中的二萜类化合物组成，赞比亚巴豆是曲洛班类化合物的重要来源。