Peeters D, Peters I R, Clercx C, Day M J
Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine, University of Liège, Sart-Tilman B44, 4000 Liège, Belgium.
Vet Immunol Immunopathol. 2006 Mar 15;110(1-2):65-77. doi: 10.1016/j.vetimm.2005.09.004. Epub 2005 Oct 13.
Idiopathic canine eosinophilic bronchopneumopathy (EBP) is a disease characterized by eosinophilic infiltration of the pulmonary interstitium and bronchial mucosa, a cause for which has not yet been discovered. A recent study, examining the relative proportion of various lymphocyte cell subsets within bronchoalveolar lavage fluid from dogs with EBP, has shown a selective increase in CD4(+) T-cells and a selective decrease in CD8(+) T-cells, suggesting that a similar Th2 immune response might occur in EBP. The aim of the present study was to determine the profile of cytokine, chemokine and CC chemokine receptor 3 (CCR3) messenger RNA (mRNA) expression in bronchial tissue from dogs with EBP. Real-time RT-PCR assays were used for the quantification of mRNA encoding for a panel of cytokines, CC chemokines and CCR3 in perendoscopic bronchial biopsies from eight dogs with EBP and seven age-matched control dogs. Messenger RNA transcribed from the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase was used for normalisation of the threshold cycle in order to determine the relative copy numbers of the transcripts. No significant difference in the expression of any cytokine, MCP-1, -2, -4 and CCR3 was found between control and EBP dogs. The expression of transcript for MCP-3, eotaxin-2 and -3 was significantly greater in bronchial biopsies from dogs with EBP than in samples from control dogs while there was significantly less mRNA encoding RANTES in the mucosa of dogs with EBP. In conclusion, the cytokine mRNA expression profile in perendoscopic bronchial biopsies is similar in dogs with EBP and dogs without respiratory disease. Further studies on the quantification of mRNA encoding cytokines in isolated T lymphocytes from bronchoalveolar lavage fluid or bronchial biopsies are needed before any conclusion on the cytokine profile in canine EBP can be drawn. Eotaxin-2, -3 and MCP-3 appear to be implicated in the pathogenesis of the disease.
特发性犬嗜酸性粒细胞性支气管肺炎（EBP）是一种以肺间质和支气管黏膜嗜酸性粒细胞浸润为特征的疾病，其病因尚未明确。最近一项研究检测了EBP犬支气管肺泡灌洗液中各种淋巴细胞亚群的相对比例，结果显示CD4(+) T细胞选择性增加，CD8(+) T细胞选择性减少，这表明EBP可能发生了类似的Th2免疫反应。本研究的目的是确定EBP犬支气管组织中细胞因子、趋化因子和CC趋化因子受体3（CCR3）信使核糖核酸（mRNA）的表达谱。采用实时逆转录聚合酶链反应（RT-PCR）检测8只EBP犬和7只年龄匹配的对照犬经内镜支气管活检组织中一组细胞因子、CC趋化因子和CCR3编码mRNA的量。以内参基因甘油醛-3-磷酸脱氢酶转录的信使核糖核酸标准化阈值循环，以确定转录本的相对拷贝数。对照犬和EBP犬在任何细胞因子、MCP-1、-2、-4和CCR3的表达上均无显著差异。EBP犬支气管活检组织中MCP-3、嗜酸性粒细胞趋化因子-2和-3的转录本表达明显高于对照犬，而EBP犬黏膜中RANTES编码的信使核糖核酸明显减少。总之，EBP犬经内镜支气管活检组织中的细胞因子信使核糖核酸表达谱与无呼吸道疾病的犬相似。在得出犬EBP细胞因子谱的任何结论之前，需要进一步研究支气管肺泡灌洗液或支气管活检组织中分离的T淋巴细胞中细胞因子编码信使核糖核酸的定量。嗜酸性粒细胞趋化因子-2、-3和MCP-3似乎与该疾病的发病机制有关。
