Onken Michael D, Lin Amy Y, Worley Lori A, Folberg Robert, Harbour J William
Department of Ophthalmology & Visual Sciences, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Am J Ophthalmol. 2005 Oct;140(4):748-9. doi: 10.1016/j.ajo.2005.04.024.
To determine whether there is an association between gene expression profile and looping extravascular matrix patterns in primary uveal melanomas.
Laboratory investigation.
Formalin-fixed, paraffin-embedded sections from 22 primary uveal melanomas that previously were analyzed by microarray gene expression profiling were stained for periodic acid-Schiff and scored in a masked fashion for the presence of looping extravascular matrix patterns.
A strong association was observed between looping patterns and the unfavorable class 2 molecular prognostic signature (P < .001).
Looping extravascular matrix patterns occur almost exclusively in class 2 uveal melanomas. This finding may provide new insights into the pathogenesis and management of uveal melanoma.
确定原发性葡萄膜黑色素瘤的基因表达谱与血管外基质环状模式之间是否存在关联。
实验室研究。
对22例原发性葡萄膜黑色素瘤的福尔马林固定、石蜡包埋切片进行过碘酸-希夫染色，并以盲法对血管外基质环状模式的存在情况进行评分，这些切片之前已通过微阵列基因表达谱分析。
观察到环状模式与不良的2类分子预后特征之间存在强关联（P <.001）。
血管外基质环状模式几乎仅出现在2类葡萄膜黑色素瘤中。这一发现可能为葡萄膜黑色素瘤的发病机制和治疗提供新的见解。
