Bente Dennis A, Melkus Michael W, Garcia J Victor, Rico-Hesse Rebeca
Department of Virology and Immunology, Southwest Foundation for Biomedical Research, 7620 NW Loop 410, San Antonio, TX 78227, USA.
J Virol. 2005 Nov;79(21):13797-9. doi: 10.1128/JVI.79.21.13797-13799.2005.
The increased transmission and geographic spread of dengue fever (DF) and its more severe presentation, dengue hemorrhagic fever (DHF), make it the most important mosquito-borne viral disease of humans (50 to 100 million infections/year) (World Health Organization, Fact sheet 117, 2002). There are no vaccines or treatment for DF or DHF because there are no animal or other models of human disease; even higher primates do not show symptoms after infection (W. F. Scherer, P. K. Russell, L. Rosen, J. Casals, and R. W. Dickerman, Am. J. Trop. Med. Hyg. 27:590-599, 1978). We demonstrate that nonobese diabetic/severely compromised immunodeficient (NOD/SCID) mice xenografted with human CD34+ cells develop clinical signs of DF as in humans (fever, rash, and thrombocytopenia), when infected in a manner mimicking mosquito transmission (dose and mode). These results suggest this is a valuable model with which to study pathogenesis and test antidengue products.
登革热（DF）的传播增加及其地理范围的扩大，以及其更为严重的表现形式登革出血热（DHF），使其成为人类最重要的蚊媒病毒性疾病（每年感染5000万至1亿例）（世界卫生组织，情况说明书第117号，2002年）。由于没有人类疾病的动物模型或其他模型，因此没有针对登革热或登革出血热的疫苗或治疗方法；即使是高等灵长类动物在感染后也不会出现症状（W.F.谢勒、P.K.拉塞尔、L.罗森、J.卡萨尔斯和R.W.迪克曼，《美国热带医学与卫生杂志》27:590 - 599，1978年）。我们证明，用人CD34 + 细胞异种移植的非肥胖糖尿病/严重免疫缺陷（NOD/SCID）小鼠，在以模拟蚊子传播的方式（剂量和模式）感染时，会出现与人类登革热相同的临床症状（发热、皮疹和血小板减少）。这些结果表明，这是一个研究发病机制和测试抗登革热产品的有价值的模型。
