通过多位点酶电泳对丹麦单核细胞增生李斯特菌分离株进行特征分析。
Characterisation of Danish isolates of Listeria monocytogenes by multilocus enzyme electrophoresis.
作者信息
Nørrung B
机构信息
Institute of Veterinary Microbiology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
出版信息
Int J Food Microbiol. 1992 Jan-Feb;15(1-2):51-9. doi: 10.1016/0168-1605(92)90135-p.
A total of 84 strains of Listeria monocytogenes were analysed by multilocus enzyme electrophoresis at twelve enzyme loci. Eight enzyme loci were polymorphic with between 2 and 4 alleles per locus. Fourteen electrophoretic types (ETs) were identified. Among 62 human clinical isolates from Denmark, 8 different ETs were defined. Two ETs, designated ET 1 and ET 6, accounted for 77% of the human clinical isolates investigated. These ETs are identical with those responsible for several epidemics in Switzerland and in the United States. Comparison of 58 isolates of L. monocytogenes, typed by MEE, in relation to phage typing showed that phage typing was more discriminatory than MEE. The ability of MEE to distinguish between phage types of Epi-type and other phage types, however, was almost optimal. MEE typed 23 of 24 strains of Epi-type as belonging to ET 1. In contrast ET 1 was not found in 26 strains with phage types other than the Epi-type.
共对84株单核细胞增生李斯特菌进行了多位点酶电泳分析，检测了12个酶位点。其中8个酶位点具有多态性，每个位点有2至4个等位基因。共鉴定出14种电泳类型（ETs）。在来自丹麦的62株人类临床分离株中，定义了8种不同的ETs。其中两种ETs，即ET 1和ET 6，占所研究人类临床分离株的77%。这些ETs与在瑞士和美国引发几次疫情的ETs相同。对58株经多位点酶电泳分型的单核细胞增生李斯特菌分离株进行噬菌体分型比较，结果表明噬菌体分型比多位点酶电泳更具鉴别力。然而，多位点酶电泳区分流行株型噬菌体类型和其他噬菌体类型的能力几乎是最佳的。多位点酶电泳将24株流行株型中的23株分型为ET 1。相比之下，在26株非流行株型噬菌体类型的菌株中未发现ET 1。
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