Chung Young, Klimanskaya Irina, Becker Sandy, Marh Joel, Lu Shi-Jiang, Johnson Julie, Meisner Lorraine, Lanza Robert
Advanced Cell Technology, Worcester, Massachusetts 01605, USA.
Nature. 2006 Jan 12;439(7073):216-9. doi: 10.1038/nature04277. Epub 2005 Oct 16.
The most basic objection to human embryonic stem (ES) cell research is rooted in the fact that ES cell derivation deprives embryos of any further potential to develop into a complete human being. ES cell lines are conventionally isolated from the inner cell mass of blastocysts and, in a few instances, from cleavage stage embryos. So far, there have been no reports in the literature of stem cell lines derived using an approach that does not require embryo destruction. Here we report an alternative method of establishing ES cell lines-using a technique of single-cell embryo biopsy similar to that used in pre-implantation genetic diagnosis of genetic defects-that does not interfere with the developmental potential of embryos. Five putative ES and seven trophoblast stem (TS) cell lines were produced from single blastomeres, which maintained normal karyotype and markers of pluripotency or TS cells for up to more than 50 passages. The ES cells differentiated into derivatives of all three germ layers in vitro and in teratomas, and showed germ line transmission. Single-blastomere-biopsied embryos developed to term without a reduction in their developmental capacity. The ability to generate human ES cells without the destruction of ex utero embryos would reduce or eliminate the ethical concerns of many.
对人类胚胎干细胞（ES细胞）研究最基本的反对意见源于这样一个事实，即ES细胞的获取剥夺了胚胎进一步发育成完整人类的任何潜力。ES细胞系通常从囊胚的内细胞团中分离出来，在少数情况下，从卵裂期胚胎中分离。到目前为止，文献中还没有关于使用不要求破坏胚胎的方法获得干细胞系的报道。在此，我们报告一种建立ES细胞系的替代方法——使用一种类似于用于遗传缺陷植入前基因诊断的单细胞胚胎活检技术——该方法不会干扰胚胎的发育潜力。从单个卵裂球产生了5个假定的ES细胞系和7个滋养层干细胞（TS细胞）系，这些细胞系在多达50多代中维持了正常的核型以及多能性或TS细胞的标志物。ES细胞在体外和畸胎瘤中分化为所有三个胚层的衍生物，并显示出种系传递。经单细胞活检的胚胎发育至足月，其发育能力没有降低。在不破坏体外胚胎的情况下产生人类ES细胞的能力将减少或消除许多伦理担忧。
