佛波醇肉豆蔻酸酯乙酸酯刺激的多形核中性粒细胞中CD11b下调的机制。
Mechanism of CD11b down-regulation from phorbol myristate acetate stimulated polymorphonuclear neutrophils.
作者信息
Aziz Khalil A
机构信息
Regional Department of Immunology, Heart of England NHS Foundation, Birmingham, United Kingdom.
出版信息
Saudi Med J. 2005 Oct;26(10):1596-601.
OBJECTIVE
To investigate the mechanism of CD11b down-regulation in phorbol myristate acetate (PMA) stimulated polymorphonuclear leukocytes (PMN).
METHODS
Purified PMN were stimulated with PMA in the presence, or absence, of various enzyme inhibitors. Following stimulation, PMN CD11b expression was examined by flow cytometry and Western-blotting. The entire work was carried out at Liverpool University between the period of 1998 and 2001.
RESULTS
Stimulation of PMN with PMA induced the down-regulation of CD11b by a mechanism involving a combination of an oxidant and a serine protease; most likely the primary granule derived elastase.
CONCLUSION
The present study shows for the first time the cooperation of both oxidants and enzymes in the down-regulation of PMN adhesion receptors.
目的
研究佛波酯（PMA）刺激多形核白细胞（PMN）后CD11b下调的机制。
方法
在存在或不存在各种酶抑制剂的情况下，用PMA刺激纯化的PMN。刺激后，通过流式细胞术和蛋白质印迹法检测PMN CD11b的表达。整个研究工作于1998年至2001年在利物浦大学进行。
结果
PMA刺激PMN通过一种涉及氧化剂和丝氨酸蛋白酶（最可能是源自初级颗粒的弹性蛋白酶）组合的机制诱导CD11b下调。
结论
本研究首次表明氧化剂和酶在PMN黏附受体下调中的协同作用。
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