Turner S Fiona, Clarke Stuart M, Rennie Adrian R, Thirtle Paul N, Li Zhi Xin, Thomas Robert K, Langridge Sean, Penfold Jeffery
Faculty of Life Sciences, University of Manchester, Sackville Street, Manchester M60 1QD, UK.
Langmuir. 2005 Oct 25;21(22):10082-8. doi: 10.1021/la050256o.
The technique of neutron reflection has been used to investigate the adsorption of alpha-enriched gelatin from aqueous solution onto spun polystyrene substrates. Neutron reflection can provide information about the distribution of material perpendicular to an interface as well as total adsorbed amounts. The adsorbed layers were found to have maximum density at the surface, decaying with distance into solution. The adsorbed amount, layer thickness, and density were all seen to increase with solution concentration. Temperature was found to have little effect on adsorption. Thicker, less dense layers were observed at high pH and thinner, denser layers were observed at low pH, but the total adsorbed amount did not change significantly. The presence of sodium chloride had little effect on the adsorbed layers. The results are discussed in the context of other studies and the known amino acid sequence of alpha-gelatin.
