Fast Jonas, Mossberg Ann-Kristin, Nilsson Hanna, Svanborg Catharina, Akke Mikael, Linse Sara
Department of Biophysical Chemistry, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.
FEBS Lett. 2005 Nov 7;579(27):6095-100. doi: 10.1016/j.febslet.2005.08.089. Epub 2005 Oct 6.
HAMLET (human alpha-lactalbumin made lethal to tumor cells) is a complex between alpha-lactalbumin and oleic acid that induces apoptosis in tumor cells, but not in healthy cells. Heteronuclear nuclear magnetic resonance (NMR) spectroscopy was used to determine the structure of 13C-oleic acid in HAMLET, and to study the 15N-labeled protein. Nuclear Overhauser enhancement spectroscopy shows that the two ends of the fatty acid are in close proximity and close to the double bond, indicating that the oleic acid is bound to HAMLET in a compact conformation. The data further show that HAMLET is a partly unfolded/molten globule-like complex under physiological conditions.
哈姆雷特（对肿瘤细胞具有致死性的人α-乳白蛋白）是α-乳白蛋白与油酸形成的复合物，可诱导肿瘤细胞凋亡，但对健康细胞无此作用。采用异核核磁共振（NMR）光谱法测定了哈姆雷特中13C-油酸的结构，并研究了15N标记的蛋白质。核Overhauser增强光谱表明，脂肪酸的两端靠得很近且靠近双键，这表明油酸以紧密构象与哈姆雷特结合。数据进一步表明，哈姆雷特在生理条件下是一种部分展开/类熔球状复合物。
