Paul B, Pfister W, Wutzler P, Gängler P
Institut für Medizinische Mikrobiologie, Medizinische Akademie, Erfurt, Bundesrepublik Deutschland.
Dtsch Zahn Mund Kieferheilkd Zentralbl. 1992;80(1):7-12.
The dental plaque of 15 patients with gingivitis and of 15 patients with marginal periodontitis was won by means of two different methods of isolation and sampling. Either the plaque was sampled by a scaler with and without covering the crown. The estimation of plaque-microflora was done by microscopical and cultural bacteriological methods. Differences in the composition of plaque-microflora could be found in dependence of method used in isolation and sampling. Also differences in bacterial colonisation of different regions were found using the same technique of sampling. The subgingival plaque in marginal periodontitis was different of the plaque in gingivitis. In plaque-microflora of periodontitis the percentage of gram-positive cocci and rods was decreasing, the percentage of gram-negative and mobile bacteria was increasing compared with the bacterial flora in gingivitis. In the subgingival plaque in gingivitis streptococci and actinomyces were the predominant bacteria.
通过两种不同的隔离和采样方法获取了15名牙龈炎患者和15名边缘性牙周炎患者的牙菌斑。牙菌斑要么用刮治器采样，采样时牙冠有覆盖和无覆盖两种情况。通过显微镜和培养细菌学方法对菌斑微生物群进行评估。根据隔离和采样所使用的方法，可以发现菌斑微生物群组成存在差异。使用相同的采样技术，还发现了不同区域细菌定植的差异。边缘性牙周炎的龈下菌斑与牙龈炎的菌斑不同。与牙龈炎的细菌菌群相比，牙周炎菌斑微生物群中革兰氏阳性球菌和杆菌的百分比在下降，革兰氏阴性和可移动细菌的百分比在增加。在牙龈炎的龈下菌斑中，链球菌和放线菌是主要细菌。
