利用高级回交和分子标记辅助选择在水稻（Oryza sativa L.）中培育单片段代换系（SSSLs）

[Developing single segment substitution lines (SSSLs) in rice (Oryza sativa L.) using advanced backcrosses and MAS].

作者信息

He Feng-Hua, Xi Zhang-Ying, Zeng Rui-Zhen, Talukdar Akshay, Zhang Gui-Quan

机构信息

Guangdong Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou 510642, China.

出版信息

Yi Chuan Xue Bao. 2005 Aug;32(8):825-31.

PMID:16231737

Abstract

A novel population consisted of 86 single segment substitution lines (SSSLs) were developed from advanced backcrosses between the recipient, Huajingxian 74 and six donors by microsatellite marker-assisted selection (MAS). Fifty-two SSSLs were selected in BC3F2, and 34 others were selected in BC3F3. Every SSSL contains a single chromosome segment introgressed from one donor on the genetic background of Huajingxian 74. The substituted segments in SSSLs were distributed on 12 rice chromosomes. The estimated length of the substituted segments in SSSLs ranged from 1.5 cM to 56.3 cM with an average of 23.0 cM. Total of the substituted segments covered 57.1% of the rice genome.

摘要

通过微卫星标记辅助选择（MAS），从受体华粳籼74与六个供体的高级回交后代中培育出了一个由86个单片段代换系（SSSL）组成的新型群体。在BC3F2中选择了52个SSSL，在BC3F3中选择了另外34个。每个SSSL在华粳籼74的遗传背景上包含一个从一个供体渗入的单染色体片段。SSSL中的代换片段分布在12条水稻染色体上。SSSL中代换片段的估计长度在1.5 cM至56.3 cM之间，平均为23.0 cM。代换片段的总和覆盖了水稻基因组的57.1%。