Xi Zhang-Ying, He Feng-Hua, Zeng Rui-Zhen, Zhang Ze-Min, Ding Xiao-Hua, Li Wen-Tao, Zhang Gui-Quan
Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, PR China.
Genome. 2006 May;49(5):476-84. doi: 10.1139/g06-005.
Naturally occurring allelic variations underlying complex traits are useful resources for the functional analysis of plant genes. To facilitate the genetic analysis of complex traits and the use of marker-assisted breeding in rice, we developed a wide population consisting of 217 chromosome single-segment substitution lines (SSSLs) using Oryza sativa L. 'Hua-Jing-Xian74' (HJX74), an elite Indica cultivar, as recipient, and 6 other accessions, including 2 Indica and 4 Japonica, as donors. Each SSSL contains a single substituted chromosome segment derived from 1 of the 6 donors in the genetic background of HJX74. The total size of the substituted segments in the SSSL population was 4695.0 cM, which was 3.1 times that of rice genome. To evaluate the potential application of these SSSLs for quantitative trait loci detection, phenotypic variations of the quantitative traits of days to heading and grain length in the population consisting of 210 SSSLs were observed under natural environmental conditions. The results demonstrated that there was a wide range of phenotypic variation in the traits in the SSSL population. These genetic materials will be powerful tools to dissect complex traits into a set of monogenic loci and to assign phenotypic values to different alleles at the locus of interest.
复杂性状背后的自然发生的等位基因变异是植物基因功能分析的有用资源。为了促进水稻复杂性状的遗传分析和标记辅助育种的应用，我们以优良籼稻品种‘华粳籼74’（HJX74）为受体，包括2个籼稻品种和4个粳稻品种在内的其他6份材料为供体，构建了一个由217个染色体单片段代换系（SSSL）组成的群体。每个SSSL在HJX74的遗传背景中包含一个来自6个供体之一的单代换染色体片段。SSSL群体中代换片段的总长度为4695.0 cM，是水稻基因组长度的3.1倍。为了评估这些SSSL在数量性状基因座检测中的潜在应用，在自然环境条件下观察了由210个SSSL组成的群体中抽穗天数和粒长等数量性状的表型变异。结果表明，SSSL群体中这些性状存在广泛的表型变异。这些遗传材料将成为将复杂性状分解为一系列单基因座，并为目标基因座的不同等位基因赋予表型值的有力工具。
