酿酒酵母在葡萄糖的需氧和厌氧分批发酵中糠醛的转化

Conversion of furfural in aerobic and anaerobic batch fermentation of glucose by Saccharomyces cerevisiae.

作者信息

Taherzadeh M J, Gustafsson L, Niklasson C, Lidén G

机构信息

Department of Chemical Reaction Engineering, Chalmers University of Technology, S-412 96 Göteborg, Sweden.

出版信息

J Biosci Bioeng. 1999;87(2):169-74. doi: 10.1016/s1389-1723(99)89007-0.

DOI:10.1016/s1389-1723(99)89007-0

PMID:16232445

Abstract

The effect of furfural on aerobic and anaerobic batch cultures of Saccharomyces cerevisiae CBS 8066 growing on glucose was investigated. Furfural was found to decrease both the specific growth rate and ethanol production rate after pulse additions in both anaerobic and aerobic batch cultures. The specific growth rate remained low until the furfural had been completely consumed, and then increased somewhat, but not to the initial value. The CO(2) evolution rate decreased to about 35% of the value before the addition of 4 g x l(-1) furfural, in both aerobic and anaerobic fermentations. The decrease of the CO(2) evolution rate was rapid at first, and then a more gradual decrease was observed. The furfural was converted mainly to furfuryl alcohol, with a specific conversion rate of 0.6 (+/-0.03) g (furfural) x g(-1) (biomass) x h(-1) by exponentially growing cells. However, the conversion rate of furfural by cells in the stationary phase was much lower. A previously unidentified compound was detected during the conversion of furfural. This compound was characterized by mass spectrometry and it is suggested that it is formed from furfural and pyruvate.

摘要

研究了糠醛对在葡萄糖上生长的酿酒酵母CBS 8066好氧和厌氧分批培养的影响。发现在厌氧和好氧分批培养中脉冲添加糠醛后，其会降低比生长速率和乙醇生产率。在糠醛完全消耗之前，比生长速率一直保持较低水平，然后有所增加，但未恢复到初始值。在好氧和厌氧发酵中，CO₂ 释放速率降至添加4 g·L⁻¹ 糠醛之前值的约35%。CO₂ 释放速率起初迅速下降，随后下降更为平缓。糠醛主要转化为糠醇，指数生长的细胞对糠醛的比转化率为0.6（±0.03）g（糠醛）·g⁻¹（生物量）·h⁻¹。然而，静止期细胞对糠醛的转化率要低得多。在糠醛转化过程中检测到一种先前未鉴定的化合物。通过质谱对该化合物进行了表征，推测其由糠醛和丙酮酸形成。