Chartrain M, Ikemoto N, Taylor C, Stahl S, Sandford V, Gbewonyo K, Chirdo C, Maxwell C, Osoria J, Buckland B, Greasham R
Department of Bioprocess, Merck Research Laboratories, RY 80Y-105, Merck and Co., PO Box 2000, Rayway, NJ 07065, USA.
J Biosci Bioeng. 2000;90(3):321-7.
An attractive method for producing cis-1,2-dihydroxy-3-methylcyclohexa-3,5-diene (toluene cis glycol) was developed employing a cis dihydrodiol dehydrogenase "deficient" strain of Rhodococcus (MA 7249). The toluene cis glycol produced was found to have optical rotations of [alpha]D25 = +25.8 (c 0.45, CH3OH) and +72.8 (c 0.42, CHCl3) which indicated an absolute configuration of (1S,2R) when compared with previously published values. When cultivated in laboratory fermentor in the presence of toluene vapors, MA 7249 reached a toluene cis glycol concentration up to 18 g/l in 110 h. Culture MA 7249 also accumulated cis (1S,2R) dihydrodiols from dihydronaphthalene, biphenyl, chlorobenzene, and styrene.
利用一株红球菌（MA 7249）的顺式二氢二醇脱氢酶“缺陷型”菌株，开发出了一种制备顺式-1,2-二羟基-3-甲基环己-3,5-二烯（甲苯顺式二醇）的有效方法。所制备的甲苯顺式二醇的旋光度为[α]D25 = +25.8（c 0.45，CH3OH）和 +72.8（c 0.42，CHCl3），与先前公布的值相比，这表明其绝对构型为(1S,2R)。当在实验室发酵罐中在甲苯蒸气存在下培养时，MA 7249在110小时内达到了高达18 g/l的甲苯顺式二醇浓度。培养物MA 7249还从二氢萘、联苯、氯苯和苯乙烯中积累了顺式(1S,2R)二氢二醇。
