Szabó G, Schablik M, Fekete Z, Zsindely A
Acta Biol Acad Sci Hung. 1978;29(4):375-84.
Inositolless (inl-) Neurospora crassa strains were treated with DNA (allo-DNA) of wild type N. Crassa. Hyphal fragments of a mycelial suspension of the N. Crassa ragged inl- strain used as recipient in our transformation experiments were found to consist of units containing 100--1000 nuclei. In this strain the inositol-independent (inl+) nuclei appearing after DNA treatment or by spontaneous reversion are present in the cytoplasm together with a large number of inl- nuclei. Thus, both transformation and reversion initially must result in heterokaryosis. Under appropriate conditions the inl- nuclei can be detected in the transformed and spontaneous inl+ phenotype revertant strains. Spontaneous revertants are usually characterized by the loss of their inl+ nuclei after transfers on inositol-supplemented medium. On minimal medium, the growth rate of transformed strains is significantly lower than that of spontaneous revertants. The inl+ gene appearing after DNA treatment or by spontaneous reversion is inherited as a trait bound to chromosomes. In crosses with the transformed strains, there is a significant increase in the number of non-Mendelian (6:2 and 5:3) tetrads in the inl locus.
用野生型粗糙脉孢菌的DNA（异体DNA）处理无肌醇（inl-）粗糙脉孢菌菌株。在我们的转化实验中用作受体的粗糙脉孢菌粗糙inl-菌株的菌丝悬浮液的菌丝片段被发现由含有100-1000个细胞核的单位组成。在该菌株中，DNA处理后或通过自发回复出现的不依赖肌醇（inl+）的细胞核与大量inl-细胞核一起存在于细胞质中。因此，转化和回复最初都必须导致异核体形成。在适当条件下，可以在转化的和自发的inl+表型回复菌株中检测到inl-细胞核。自发回复突变体通常的特征是在添加肌醇的培养基上转移后其inl+细胞核丢失。在基本培养基上，转化菌株的生长速率明显低于自发回复突变体。DNA处理后或通过自发回复出现的inl+基因作为与染色体相关的性状遗传。在与转化菌株的杂交中，inl位点的非孟德尔（6:2和5:3）四分体数量显著增加。
