Yu W, Sato K, Wakabayashi M, Nakaishi T, Ko-Mitamura E P, Shima Y, Urabe I, Yomo T
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
J Biosci Bioeng. 2001;92(6):590-3. doi: 10.1263/jbb.92.590.
The liposome consisting of eggPC, cholesterol, and DSPE-PEG5000 with a molar ratio of 1.5:1:0.08 was used to entrap cell-free protein synthesis reaction mixture. The synthesis of a mutant green fluorescent protein in the liposome was confirmed by the fluorescence emitted from the liposome on flow cytometry analysis and fluorescence microscopy. The protein synthesized in the liposome is hence functional.
由摩尔比为1.5:1:0.08的蛋黄卵磷脂、胆固醇和二硬脂酰磷脂酰乙醇胺-聚乙二醇5000组成的脂质体用于包裹无细胞蛋白质合成反应混合物。通过流式细胞术分析和荧光显微镜观察脂质体发出的荧光，证实了脂质体内突变绿色荧光蛋白的合成。因此，脂质体内合成的蛋白质具有功能。
