Kanagawa Takahiro
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
J Biosci Bioeng. 2003;96(4):317-23. doi: 10.1016/S1389-1723(03)90130-7.
Polymerase chain reaction (PCR) is often used for the amplification of a mixture of homologous genes. PCR bias and artifact formation can occur in multitemplate PCR, and provide incorrect information on the abundance and diversity of genes. PCR bias and artifact formation occur at a higher rate during the last few cycles of the reaction, and therefore can be avoided by stopping the PCR earlier.
聚合酶链反应（PCR）常用于扩增同源基因混合物。在多模板PCR中可能会出现PCR偏差和假象形成，从而提供有关基因丰度和多样性的错误信息。PCR偏差和假象形成在反应的最后几个循环中发生率较高，因此可以通过提前终止PCR来避免。
