宏基因组学或宏分类学：揭示鼻窦微生物组的最佳实践方法
Metagenomics or Metataxonomics: Best Practice Methods to Uncover the Sinus Microbiome.
作者信息
Burdon Isabella, Bouras George, Fenix Kevin, Yeo Kenny, Connell James, Cooksley Clare, Barry Emma, Vreugde Sarah, Wormald Peter J, Psaltis Alkis J
机构信息
Department of Surgery, Otolaryngology Head and Neck Surgery, University of Adelaide, Adelaide, South Australia, Australia.
Department of Otolaryngology, The Basil Hetzel Institute for Translational Research, Woodville South, South Australia, Australia.
出版信息
Int Forum Allergy Rhinol. 2025 Sep;15(9):989-992. doi: 10.1002/alr.23617. Epub 2025 May 30.
Long-read-metagenomic sequencing is the best method for analyzing the sinus microbiome. 16s-rRNA-sequencing (both long and short read) results in PCR amplification bias that significantly distorts the sinus microbiome.
长读长宏基因组测序是分析鼻窦微生物组的最佳方法。16S rRNA测序（长读长和短读长）会导致PCR扩增偏差，从而严重扭曲鼻窦微生物组。
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