Ballance Simon, Holtan Synnøve, Aarstad Olav Andreas, Sikorski Pawel, Skjåk-Braek Gudmund, Christensen Bjørn E
Norwegian Biopolymer Laboratory (NOBIPOL), Department of Biotechnology, Norwegian University of Science and Technology (NTNU), Sem Saelands vei 6/8, N-7491 Trondheim, Norway.
J Chromatogr A. 2005 Nov 4;1093(1-2):59-68. doi: 10.1016/j.chroma.2005.07.051. Epub 2005 Aug 18.
Alginates comprised of essentially alternating units of mannuronic (M) acid-guluronic (G) acid (MG-alginate), and G-blocks isolated from a seaweed where subjected to partial acid hydrolysis at pH 3.5 The chain-length distribution of oligosaccharides in the hydrolysate were investigated by statistical analysis after their separation with high-performance anion-exchange chromatography and pulsed amperometric detection (HPAEC-PAD). Simulated depolymerisation of the MG-alginate provided an estimate of the ratio between two acid hydrolysis rate constants (p=8.3+/-1) and the average distribution of the MM linkages in the original sample of polysaccharide chains. In conclusion, we found HPAEC-PAD together with statistical analysis was a useful method to investigate the fine structure and some properties of binary polysaccharides.
由甘露糖醛酸（M）-古洛糖醛酸（G）基本交替单元组成的藻酸盐（MG-藻酸盐），以及从海藻中分离出的G-嵌段，在pH 3.5条件下进行部分酸水解。水解产物中低聚糖的链长分布在通过高效阴离子交换色谱和脉冲安培检测（HPAEC-PAD）分离后进行统计分析。MG-藻酸盐的模拟解聚提供了两个酸水解速率常数之间的比率估计值（p = 8.3±1）以及多糖链原始样品中MM键的平均分布。总之，我们发现HPAEC-PAD与统计分析相结合是研究二元多糖精细结构和一些性质的有用方法。
