Bignami A, Asher R, Perides G
Department of Pathology, Harvard Medical School, West Roxbury Department of Veterans Affairs Medical Center, Boston, MA 02132.
Brain Res. 1992 May 1;579(1):173-7. doi: 10.1016/0006-8993(92)90759-3.
The distribution of hyaluronate (HA) and chondroitin sulfate (CS) proteoglycan in the rat cerebral cortex was compared. For the localization of HA, the sections were incubated with human glial hyaluronate-binding protein (GHAP) and then reacted with monoclonal or polyclonal antibodies to GHAP. Polyclonal antibodies raised in rabbit were used for double-labeling experiments with monoclonal antibodies raised in mice and reacting with CS proteoglycans. Little reactivity was observed in rat cerebral cortex with polyclonal GHAP antibodies if the sections were not incubated with GHAP. Monoclonal antibodies to GHAP did not react with murine tissues. CS proteoglycans were localized in chondroitinase-digested sections with monoclonal antibodies reacting with the 4-sulfated oligosaccharide stubs formed by the digestion with chondroitinase ABC of CS side chains. In the rat cerebral cortex, the distribution of CS proteoglycans was similar to that reported by Bertolotto, A., Rocca, G. and Schiffer, D., J. Neurol. Sci., 100 (1990) 113-123, and his collaborators using the same antibodies. Many neurons mainly located in the upper and deep cortical layers were surrounded by CS immunoreactive material. Several (but not all) CS-positive neurons also stained for HA with an identical distribution except that in most instances the staining was confined to the periphery of the perikaryon and did not extend to the dendritic tree. The finding suggests that cerebral cortex CS proteoglycan is capable of interacting with HA.
比较了大鼠大脑皮质中透明质酸盐（HA）和硫酸软骨素（CS）蛋白聚糖的分布情况。为了定位HA，将切片与人神经胶质透明质酸结合蛋白（GHAP）孵育，然后与抗GHAP的单克隆或多克隆抗体反应。用兔产生的多克隆抗体与小鼠产生的、与CS蛋白聚糖反应的单克隆抗体进行双重标记实验。如果切片不与GHAP孵育，在大鼠大脑皮质中用多克隆GHAP抗体观察到的反应性较低。抗GHAP的单克隆抗体不与鼠组织反应。CS蛋白聚糖通过与经软骨素酶ABC消化CS侧链形成的4-硫酸化寡糖残端反应的单克隆抗体定位在经软骨素酶消化的切片中。在大鼠大脑皮质中，CS蛋白聚糖的分布与Bertolotto、A.、Rocca、G.和Schiffer、D.（《神经科学杂志》，100（1990）113 - 123）及其合作者使用相同抗体报道的分布相似。许多主要位于皮质上层和深层的神经元被CS免疫反应性物质包围。一些（但不是全部）CS阳性神经元也用HA染色，分布相同，只是在大多数情况下，染色局限于核周体的周边，不延伸到树突。这一发现表明大脑皮质CS蛋白聚糖能够与HA相互作用。
