Caterson B, Christner J E, Baker J R, Couchman J R
Fed Proc. 1985 Feb;44(2):386-93.
Monoclonal antibodies have been raised against determinants present in cartilage proteoglycan. Characterization of the specificity of these antibodies indicated that they recognize determinants present in the keratan sulfate glycosaminoglycan chain and on chondroitin sulfate oligosaccharide stubs attached to the proteoglycan core protein after chondroitinase digestion of the proteoglycan (i.e., delta-unsaturated 4- and 6-sulfated and unsulfated chondroitin sulfate on the proteoglycan core). The antibody recognizing keratan sulfate has been used to demonstrate the presence of a keratan sulfate-rich proteoglycan subpopulation that increases with increasing age of animal compared with chondroitin sulfate-rich proteoglycans. Monoclonal antibodies recognizing determinants on chondroitinase-treated proteoglycan have been used in immunohistochemical localization studies determining the differential distribution of 4- and 6-sulfated and unsulfated proteoglycans in tissue sections of cartilage and other noncartilaginous tissues. Digestion with chondroitinase ABC or ACII can be used to differentiate between chondroitin sulfate and dermatan sulfate proteoglycan in different connective tissues. In addition, the presence of a 6-sulfated chondroitin sulfate proteoglycan that is associated with membranes surrounding nerve and muscle fiber bundles is described. Monoclonal antibodies were also raised against the link protein(s) of cartilage proteoglycan aggregate. They have been used in peptide map analyses of link protein and in demonstrating the presence of a high-mannose oligosaccharide chain of the link proteins. The presence of high-mannose oligosaccharide structures on the link protein(s) accounts for the microheterogeneity of the link proteins (link proteins 1, 2, or 3) that is observed on sodium dodecyl sulfate-polyacrylamide gels.
已经制备出针对软骨蛋白聚糖中存在的决定簇的单克隆抗体。对这些抗体特异性的表征表明，它们识别硫酸角质素糖胺聚糖链中以及蛋白聚糖经软骨素酶消化后附着于蛋白聚糖核心蛋白上的硫酸软骨素寡糖残端中存在的决定簇（即蛋白聚糖核心上的δ-不饱和4-硫酸化、6-硫酸化和未硫酸化的硫酸软骨素）。识别硫酸角质素的抗体已被用于证明存在一个富含硫酸角质素的蛋白聚糖亚群，与富含硫酸软骨素的蛋白聚糖相比，该亚群随动物年龄增长而增加。识别软骨素酶处理的蛋白聚糖上决定簇的单克隆抗体已用于免疫组织化学定位研究，以确定软骨和其他非软骨组织切片中4-硫酸化、6-硫酸化和未硫酸化蛋白聚糖的差异分布。用软骨素酶ABC或ACII消化可用于区分不同结缔组织中的硫酸软骨素蛋白聚糖和硫酸皮肤素蛋白聚糖。此外，还描述了一种与神经和肌肉纤维束周围膜相关的6-硫酸化硫酸软骨素蛋白聚糖的存在。还制备了针对软骨蛋白聚糖聚集体连接蛋白的单克隆抗体。它们已被用于连接蛋白的肽图分析以及证明连接蛋白中高甘露糖寡糖链的存在。连接蛋白上高甘露糖寡糖结构的存在解释了在十二烷基硫酸钠-聚丙烯酰胺凝胶上观察到的连接蛋白（连接蛋白1、2或3）的微异质性。
