Liu Guo-bing, Liu Xin, Pang Zhan-jun, Xing Fu-qi
Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Di Yi Jun Yi Da Xue Xue Bao. 2005 Oct;25(10):1236-9.
To assess the significance of human papillomavirus (HPV) DNA detection in the pelvic lymph nodes in cervical cancer.
HPV L1 gene fragment was amplified by HPV-specific PCR with general consensus primers from cervical cancer tissues. The types of HPV were identified by sequencing of the PCR product. The relationship between HPV positivity and pathological findings in metastatic pelvic lymph nodes of cervical cancer was analyzed.
The positivity rate of HPV DNA was 40% in the pelvic lymph nodes in 40 cases of cervical cancer. HPV DNA was detected in the pelvic lymphatic nodes of all the 10 cases with pathologically confirmed lymph node metastasis of cervical cancer. HPV18 was detected in both of cervical and pelvic lymph nodes in some cases.
HPV DNA can be detected in the pelvic lymph nodes before pathological identification of lymph node metastasis and indicates early pelvic lymph node metastasis of cervical cancer. The presence of HPV18 often indicates high likeliness of lymph node metastasis and suggests poor prognosis of the patients.
评估人乳头瘤病毒（HPV）DNA检测在宫颈癌盆腔淋巴结中的意义。
采用通用引物通过HPV特异性PCR从宫颈癌组织中扩增HPV L1基因片段。通过PCR产物测序鉴定HPV类型。分析宫颈癌盆腔转移淋巴结中HPV阳性与病理结果之间的关系。
40例宫颈癌患者盆腔淋巴结中HPV DNA阳性率为40%。在10例经病理证实有宫颈癌淋巴结转移的患者中，均在盆腔淋巴结中检测到HPV DNA。部分病例在宫颈和盆腔淋巴结中均检测到HPV18。
在淋巴结转移的病理诊断之前，盆腔淋巴结中即可检测到HPV DNA，提示宫颈癌早期盆腔淋巴结转移。HPV18的存在常提示淋巴结转移可能性高，患者预后差。
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