Peedicayil Abraham, Sathish Narayanan, Gnanamony Manu, John Subhashini, Viswanathan Faith Rangad, Naseema Parveen Shaikh, Abraham Priya
Departments of Obstetrics & Gynaecology, Christian Medical College, Vellore, India.
J Low Genit Tract Dis. 2009 Apr;13(2):102-6. doi: 10.1097/LGT.0b013e31818c66e6.
To determine if human papillomavirus (HPV) DNA in pelvic lymph nodes or plasma of women with early-stage cervical cancer is a marker for recurrence.
Twenty-eight women undergoing radical hysterectomy for cervical cancer stage IB had HPV DNA testing in cervical tissue, plasma, and the largest lymph nodes. Human papillomavirus genotyping was done by restriction fragment length polymorphism/line blot assay. Human papillomavirus quantitation was performed with specific primers for types 16 and 18. Women were followed up to determine recurrence of disease.
Human papillomavirus DNA was detected in cervical tumor tissue from all the women. Twenty-two women had genotype 16, 5 women had HPV18, and 1 had type 31. Human papillomavirus viral load in cervical biopsies significantly correlated with the viral loads in the lymph nodes (r = 0.97; p = 0). Nine women had nodal metastasis, but only 3 of them had HPV DNA in lymph nodes. Recurrence was seen in 4 women, of whom 3 had tumor in the lymph nodes at initial surgery and none had HPV.
Human papillomavirus DNA can be detected in lymph nodes even when they are not obviously involved by tumor metastasis. This study did not show plasma or lymph node HPV DNA to be a prognostic marker.
确定早期宫颈癌女性盆腔淋巴结或血浆中的人乳头瘤病毒（HPV）DNA是否为复发标志物。
28例因IB期宫颈癌接受根治性子宫切除术的女性，对其宫颈组织、血浆及最大的淋巴结进行HPV DNA检测。采用限制性片段长度多态性/线性印迹分析法进行HPV基因分型。使用16型和18型特异性引物进行HPV定量分析。对这些女性进行随访以确定疾病复发情况。
所有女性的宫颈肿瘤组织均检测到HPV DNA。22例女性为16型基因型，5例为HPV18型，1例为31型。宫颈活检组织中的HPV病毒载量与淋巴结中的病毒载量显著相关（r = 0.97；p = 0）。9例女性有淋巴结转移，但其中仅3例淋巴结中有HPV DNA。4例女性出现复发，其中3例在初次手术时淋巴结有肿瘤，且均无HPV。
即使淋巴结未明显受肿瘤转移累及，也可检测到HPV DNA。本研究未显示血浆或淋巴结HPV DNA为预后标志物。
