一种用于汞的蓝色荧光抗体-辅助因子传感器。
A blue fluorescent antibody-cofactor sensor for mercury.
作者信息
Matsushita Masayuki, Meijler Michael M, Wirsching Peter, Lerner Richard A, Janda Kim D
机构信息
Department of Chemistry, The Scripps Research Institute, La Jolla, California 92037, USA.
出版信息
Org Lett. 2005 Oct 27;7(22):4943-6. doi: 10.1021/ol051919w.
[reaction: see text] A chemically programmed antibody sensor, consisting of a stilbenyl boronic acid cofactor and monoclonal antibody EP2-19G2, provides a new method of mercury detection. The fluorescent antibody sensor generates an intense powder blue fluorescence when bound to the stilbenyl boronic acid cofactor; however, it is quenched in the presence of Hg(2+) ions. The EP2-19G2-cofactor biosensor provides micromolar sensitivity and selectivity toward Hg(2+) ions over a wide range of metal ions in aqueous solution.
[反应：见正文] 一种由苯乙烯基硼酸辅因子和单克隆抗体EP2-19G2组成的化学编程抗体传感器，提供了一种新的汞检测方法。当与苯乙烯基硼酸辅因子结合时，荧光抗体传感器会产生强烈的粉蓝色荧光；然而，在Hg(2+)离子存在下它会被淬灭。EP2-19G2-辅因子生物传感器在水溶液中的多种金属离子范围内，对Hg(2+)离子具有微摩尔级的灵敏度和选择性。
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