Tang Bo, Ding Baiyu, Xu Kehua, Tong Lili
Engineering Research Center of Pesticide and Medicine, Intermediate Clean Production, Ministry of Education, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University, Jinan, PR China.
Chemistry. 2009;15(13):3147-51. doi: 10.1002/chem.200802165.
Seleno fluorescent probe: An organoselenium fluorescent probe (FSe-1) for mercury was designed based on the irreversible deselenation mechanism. FSe-1 exhibits an ultrahigh selectivity and sensitivity for Hg(2+) detection only for reactive selenium atom sites, due the strong affinity between Se and Hg. Furthermore, the new probe has been successfully used for imaging mercury ions in RAW 264.7 cells (a mouse macrophage cell line; see figure).Inspired by the antitoxic function of selenium towards heavy-metal ions, we designed an organoselenium fluorescent probe (FSe-1) for mercury. The reaction of FSe-1 and Hg(2+) is an irreversible deselenation mechanism based on the selenophilic character of mercury. FSe-1 exhibits an ultrahigh selectivity and sensitivity for Hg(2+) detection only for reactive selenium atom sites due to the strong affinity between Se and Hg. The experimental results proved that FSe-1 was selective for Hg(2+) ions over other relevant metal ions and bioanalytes, and also showed an enhancement in sensitivity of up to 1.0 nM, which is lower than the current Environmental Protection Agency standard for drinking water. Furthermore, the new probe has been successfully applied to the imaging of mercury ions in RAW 264.7 cells (a mouse macrophage cell line) with high sensitivity and selectivity.
基于不可逆脱硒机理设计了一种用于检测汞的有机硒荧光探针（FSe-1）。由于硒与汞之间具有很强的亲和力，FSe-1仅对活性硒原子位点的Hg(2+)检测表现出超高的选择性和灵敏度。此外，这种新型探针已成功用于RAW 264.7细胞（一种小鼠巨噬细胞系；见图）中汞离子的成像。受硒对重金属离子的解毒功能启发，我们设计了一种用于汞的有机硒荧光探针（FSe-1）。FSe-1与Hg(2+)的反应基于汞的亲硒特性，是一种不可逆的脱硒机理。由于硒与汞之间具有很强的亲和力，FSe-1仅对活性硒原子位点的Hg(2+)检测表现出超高的选择性和灵敏度。实验结果证明，FSe-1对Hg(2+)离子比对其他相关金属离子和生物分析物具有选择性，并且灵敏度提高到1.0 nM，低于美国环境保护局目前的饮用水标准。此外，这种新型探针已成功应用于RAW 264.7细胞（一种小鼠巨噬细胞系）中汞离子的成像，具有高灵敏度和选择性。
