Park Jae Eun, Lee Do Hee, Lee Jung A, Park Sung Goo, Kim Nam-Soon, Park Byoung Chul, Cho Sayeon
Systemic Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejon 305-333, Republic of Korea.
Biochem Biophys Res Commun. 2005 Dec 2;337(4):1283-7. doi: 10.1016/j.bbrc.2005.10.004. Epub 2005 Oct 10.
Angiogenesis is a complex process that is regulated by a variety of angiogenic activators and inhibitors. Disruption of the balanced angiogenesis leads to the progress of diseases such as tumor growth, rheumatoid arthritis, and various blood vessel-related disorders. Even though a number of proteins involved in angiogenesis have been identified so far, more protein factors remain to be identified due to complexity of the process. Here we report that annexin A3 (ANXA3) induces migration and tube formation of human umbilical vein endothelial cells. High level of vascular endothelial growth factor (VEGF), a prominent angiogenic factor, is also detected in conditioned medium obtained from cells transfected with ANXA3 expression plasmid. Reporter assays show that ANXA3 enhances hypoxia-inducible factor-1 (HIF-1) transactivation activity. Taken together, our results suggest that ANXA3 is a novel angiogenic factor that induces VEGF production through the HIF-1 pathway.
血管生成是一个复杂的过程，受到多种血管生成激活剂和抑制剂的调节。血管生成平衡的破坏会导致诸如肿瘤生长、类风湿性关节炎和各种血管相关疾病的进展。尽管到目前为止已经鉴定出许多参与血管生成的蛋白质，但由于该过程的复杂性，仍有更多的蛋白质因子有待鉴定。在此我们报告，膜联蛋白A3（ANXA3）可诱导人脐静脉内皮细胞迁移和形成管腔结构。在转染了ANXA3表达质粒的细胞所获得的条件培养基中也检测到高水平的血管内皮生长因子（VEGF），这是一种重要的血管生成因子。报告基因检测表明，ANXA3增强缺氧诱导因子-1（HIF-1）的反式激活活性。综上所述，我们的结果表明ANXA3是一种新型血管生成因子，它通过HIF-1途径诱导VEGF产生。
