Carvalho Sandra Elisa, Freitas Daniel, Murad André Melro, Franco Octávio Luiz, Simões-Barbosa Augusto
Universidade Católica de Brasília, Programa de Pós-graduação em Ciências Genômicas e Biotecnologia, SGAN 916, Brasília DF 70790-160, Brazil.
Exp Parasitol. 2005 Dec;111(4):260-3. doi: 10.1016/j.exppara.2005.08.007. Epub 2005 Oct 17.
This work describes the identification of a triacylglycerol lipase named TVLip directly onto blood-LB-agar plates by hemolytic screening of a Trichomonas vaginalis cDNA expression library. Sharing significant similarity in the primary sequence with other lipases, the theoretical 3D structure of the TVLip was resolved. The structure reveals the predictive conserved characteristics of other lipases from EC3.1.1.3 group, although presenting one amino acid change in the catalytic triad Ser-His-Asp. Finally, analysis of Northern blot indicates that the expression of the TVLip gene is up-regulated by iron.
这项工作描述了通过对阴道毛滴虫cDNA表达文库进行溶血筛选，直接在血LB琼脂平板上鉴定一种名为TVLip的三酰甘油脂肪酶。TVLip在一级序列上与其他脂肪酶具有显著相似性，其理论三维结构已得到解析。该结构揭示了来自EC3.1.1.3组的其他脂肪酶的预测保守特征，尽管在催化三联体Ser-His-Asp中有一个氨基酸变化。最后，Northern印迹分析表明TVLip基因的表达受铁上调。
