Modin Charlotte, Stranne Anne-Louise, Foss Morten, Duch Mogens, Justesen Jeannette, Chevallier Jacques, Andersen Lars K, Hemmersam Anne G, Pedersen Finn S, Besenbacher Flemming
Interdisciplinary Nanoscience Center (iNANO), University of Aarhus, DK-8000 Aarhus C, Denmark.
Biomaterials. 2006 Mar;27(8):1346-54. doi: 10.1016/j.biomaterials.2005.09.022. Epub 2005 Oct 19.
The quartz crystal microbalance with dissipation (QCM-D) technique was employed to characterize initial cell adhesion in terms of attachment and spreading of pre-osteoblastic MC3T3-E1 cells on Ta and Cr surfaces. Evaluation of initial cell adhesion established a correlation between input cell number and the shifts in frequency (f) and dissipation (D). The f-shift was found to be much larger in serum-free medium as compared to a medium including serum; hence, initial cell adhesion was subsequently evaluated in serum-free medium. During the first hour of adhesion, we found a positive correlation between the QCM-D f-shift and the average area of the spread cells, as measured by cryo-scanning electron microscopy (cryo-SEM). Finally, the QCM-D technique was used to study cell adhesion on different metal oxide surfaces. Initial cell adhesion on Ta was found to induce a larger f-shift as compared to Cr, indicating larger spreading of cells on Ta. Cryo-SEM data confirmed that spreading of cells on Cr was on average only two-thirds the spreading on Ta. Our results demonstrate that the QCM-D technique is a versatile technique to quickly distinguish initial cell-surface interactions on different biomaterials.
采用带耗散监测的石英晶体微天平（QCM-D）技术，从成骨前体细胞MC3T3-E1在钽（Ta）和铬（Cr）表面的附着和铺展方面来表征初始细胞黏附。对初始细胞黏附的评估建立了输入细胞数量与频率（f）和耗散（D）变化之间的相关性。结果发现，与含血清培养基相比，无血清培养基中的f变化要大得多；因此，随后在无血清培养基中评估初始细胞黏附。在黏附的第一个小时内，我们发现通过低温扫描电子显微镜（cryo-SEM）测量，QCM-D的f变化与铺展细胞的平均面积之间呈正相关。最后，利用QCM-D技术研究细胞在不同金属氧化物表面的黏附情况。结果发现，与Cr相比，细胞在Ta上的初始黏附会引起更大的f变化，表明细胞在Ta上的铺展更大。低温扫描电子显微镜数据证实，细胞在Cr上的铺展平均仅为在Ta上的三分之二。我们的结果表明，QCM-D技术是一种通用技术，可快速区分不同生物材料上的初始细胞-表面相互作用。
