Karantonis Haralabos C, Fragopoulou Elizabeth, Antonopoulou Smaragdi, Rementzis John, Phenekos Costas, Demopoulos Constantinos A
National and Kapodistrian University of Athens, Faculty of Chemistry, Panepistimioupolis, 15771 Athens, Greece.
Diabetes Res Clin Pract. 2006 Apr;72(1):33-41. doi: 10.1016/j.diabres.2005.09.003. Epub 2005 Oct 19.
The aim of our work was to carry out a randomized clinical trial with a fast-food Mediterranean type diet rich in platelet activating factor (PAF) antagonist to investigate the effect on type 2 diabetics and healthy human subject's platelet aggregation.
We extracted lipids from fast-food Mediterranean type foodstuffs, and tested them in vitro for their ability to inhibit or antagonize PAF towards washed rabbit platelets. We chose the foodstuffs that exerted the most potent in vitro anti-PAF activity and fed 22 healthy (group A) and 23 type 2 diabetics (group B) subjects on a diet containing the chosen foodstuffs. The 22 type 2 diabetics (group C) subjects were kept on their regular diet that was being followed before entering the study. Before and after a 4-week diet, all enrolled subjects underwent the following examinations; measurement of total cholesterol, low density lipoprotein (LDL-cholesterol), high density lipoprotein (HDL-cholesterol), triglycerides, glucose, HbA(1c), body mass index (BMI), and platelet aggregation in response to PAF, adenosine 5' diphosphate (ADP) and arachidonic acid (AA).
The chosen diet significantly increased the EC(50) values of PAF and ADP to groups A and B (p<0.05). No statistical difference was observed on the EC(50) value of group C. No statistical differences were detected among Cholesterol, LDL-cholesterol, triglycerides, glucose, HBA(1c), BMI, and EC(50) for AA values, for any of the three groups.
Consumption of a fast-food Mediterranean type diet rich in PAF antagonist improved platelet response of type 2 diabetics and healthy human subjects against thrombotic, inflammatory and proatherogenic factors.
我们的工作旨在开展一项随机临床试验，采用富含血小板活化因子（PAF）拮抗剂的快餐式地中海饮食，以研究其对2型糖尿病患者和健康受试者血小板聚集的影响。
我们从快餐式地中海食物中提取脂质，并在体外测试它们抑制或拮抗PAF对洗涤过的兔血小板作用的能力。我们选择了体外抗PAF活性最强的食物，并让22名健康受试者（A组）和23名2型糖尿病患者（B组）食用含有所选食物的饮食。22名2型糖尿病患者（C组）则保持进入研究前遵循的常规饮食。在为期4周的饮食前后，所有纳入的受试者均接受以下检查：总胆固醇、低密度脂蛋白（LDL-胆固醇）、高密度脂蛋白（HDL-胆固醇）、甘油三酯、葡萄糖、糖化血红蛋白（HbA1c）、体重指数（BMI）的测量以及对PAF、腺苷5'-二磷酸（ADP）和花生四烯酸（AA）反应的血小板聚集情况。
所选饮食显著提高了A组和B组对PAF和ADP的半数有效浓度（EC50）值（p<0.05）。C组EC50值未观察到统计学差异。三组中任何一组在胆固醇、LDL-胆固醇、甘油三酯、葡萄糖、糖化血红蛋白（HbA1c）、BMI以及AA的EC50值方面均未检测到统计学差异。
食用富含PAF拮抗剂的快餐式地中海饮食可改善2型糖尿病患者和健康受试者血小板对血栓形成、炎症和动脉粥样硬化形成相关因子的反应。
