Dietrich-Muszalska A, Olas B, Rabe-Jablonska J
II Department of Psychiatry, Medical University of Lodz, Poland.
Platelets. 2005 Nov;16(7):386-91. doi: 10.1080/09537100500128872.
Oxidative stress in blood platelets is observed in various diseases, including neuropsychiatric disorders. The aim of our study was to evaluate oxidative stress in blood platelets from patients with schizophrenic disorders by measuring the activity of the platelet antioxidative enzyme, superoxide dismutase (SOD), concomitant with the level of thiobarbituric acid reactive species (TBARS). In blood platelets obtained from schizophrenic patients (with paranoid schizophrenia according to DSM-IV criteria) and from healthy volunteers the level of reactive oxygen species was also measured via chemiluminescence. In resting blood platelets from schizophrenic patients the chemiluminescence was higher than in platelets from control subjects (P < 0.05), but in thrombin-activated platelets an increase (about 53%) of chemiluminescence was observed, however this increase was lower than in thrombin-stimulated platelets from healthy subjects (101.5%). The results indicate that in platelets from schizophrenic patients generation of reactive oxygen species is enhanced. Moreover, we observed that SOD activity in blood platelets from schizophrenic patients was significantly lower than in control platelets and that a correlation exists between increased lipid peroxidation and inhibition of the activity of this antioxidative enzyme in schizophrenic platelets.
在包括神经精神疾病在内的多种疾病中都观察到血小板中的氧化应激。我们研究的目的是通过测量血小板抗氧化酶超氧化物歧化酶（SOD）的活性以及硫代巴比妥酸反应性物质（TBARS）的水平，来评估精神分裂症患者血小板中的氧化应激。在从精神分裂症患者（根据DSM-IV标准诊断为偏执型精神分裂症）和健康志愿者获取的血小板中，还通过化学发光法测量了活性氧的水平。在精神分裂症患者的静息血小板中，化学发光高于对照组血小板（P < 0.05），但在凝血酶激活的血小板中，化学发光增加（约53%），然而这种增加低于健康受试者凝血酶刺激的血小板（101.5%）。结果表明，精神分裂症患者的血小板中活性氧的生成增强。此外，我们观察到精神分裂症患者血小板中的SOD活性显著低于对照血小板，并且在精神分裂症血小板中脂质过氧化增加与这种抗氧化酶活性受到抑制之间存在相关性。
