Department of General Biochemistry, Institute of Biochemistry, University of Lodz, Lodz, Poland.
Cell Biol Toxicol. 2010 Aug;26(4):355-65. doi: 10.1007/s10565-009-9148-4. Epub 2010 Jan 13.
The oxidative stress induced by acute exertion may interfere with blood platelet activation. The beneficial effect of L-carnitine (gamma-trimethylamino-beta-hydroxybutyric acid) on oxidative stress in blood platelets has not been fully investigated; however, different studies indicate that this compound modulates platelet functions. The aim of our study was to assess the effects of L-carnitine on platelet activation and oxidative/nitrative protein damage (determined by the levels of protein carbonyl groups, thiol groups, and 3-nitrotyrosine residues) in resting blood platelets or platelets treated with peroxynitrite (ONOO(-), a strong physiological oxidant) in vitro. We also investigated the effects of L-carnitine on the level of platelet glutathione and on the formation of superoxide anion radicals O2(-), lipid peroxidation measured by thiobarbituric acid reactive substances (TBARS) in blood platelets stimulated by thrombin (a strong physiological agonist), and platelet aggregation induced by adenosine diphosphate (a strong physiological stimulator). We have observed that carnitine decreases platelet activation (measured by platelet aggregation, the generation of O2(-), and TBARS production). Moreover, our results in vitro demonstrate that carnitine may protect against oxidation of thiol groups induced by ONOO(-). Thus, carnitine may have some protectory effects against oxidative changes induced in blood platelets.
急性运动引起的氧化应激可能会干扰血小板的激活。左旋肉碱（γ-三甲氨基-β-羟基丁酸）对血小板氧化应激的有益作用尚未得到充分研究；然而，不同的研究表明，这种化合物可以调节血小板的功能。我们的研究目的是评估左旋肉碱对静息血小板或用过氧亚硝酸盐（ONOO（-），一种强生理氧化剂）体外处理的血小板的血小板活化和氧化/硝化蛋白损伤（通过蛋白质羰基、巯基和 3-硝基酪氨酸残基的水平来确定）的影响。我们还研究了左旋肉碱对血小板谷胱甘肽水平的影响，以及对凝血酶（一种强生理激动剂）刺激的血小板中超氧阴离子自由基 O2（-）形成、丙二醛（TBARS）的脂质过氧化的影响，血小板聚集由二磷酸腺苷（一种强生理刺激物）诱导。我们观察到肉碱可降低血小板活化（通过血小板聚集、O2（-）的产生和 TBARS 的产生来衡量）。此外，我们的体外结果表明，肉碱可能可以防止 ONOO（-）诱导的巯基氧化。因此，肉碱可能对血小板中诱导的氧化变化具有一些保护作用。
