Farah C S, Ashman R B
Oral Biology and Pathology Research Unit, School of Dentistry, University of Queensland, Brisbane, Australia.
Oral Microbiol Immunol. 2005 Dec;20(6):376-81. doi: 10.1111/j.1399-302X.2005.00240.x.
BACKGROUND/AIMS: Clinical and laboratory studies are consistent with a major role for cell-mediated immunity in recovery from oral infection with Candida albicans, but the role of humoral immunity remains controversial. The purpose of this study was to establish the relative contributions of cellular and humoral immunity to protection against oral candidiasis in a murine model, and to determine whether host responses could be enhanced by different immunization strategies.
Active oral immunization was protective in BALB/c and CBA/CaH mice, reducing both fungal burden and duration of infection after secondary challenge, whereas systemic immunization failed to protect against subsequent oral challenge. Candida-specific IgM was the predominant antibody detected in serum following both primary and secondary oral challenge; however, Candida-specific salivary IgA was not detectable. Immunization by passive transfer of either lymphocytes or immune serum did not confer any significant protection against oral infection in either susceptible or resistant mouse strain.
The data demonstrate a possible role for mucosa-associated immunity following active immunization by the oral route, most likely exerted by local T lymphocytes resident in the oral mucosa, but there was no evidence to support a role for humoral immunity in protection against oral candidiasis.
背景/目的：临床和实验室研究均表明，细胞介导的免疫在白色念珠菌口腔感染的恢复过程中起主要作用，但体液免疫的作用仍存在争议。本研究的目的是确定细胞免疫和体液免疫在小鼠模型中对口腔念珠菌病的保护作用的相对贡献，并确定不同的免疫策略是否能增强宿主反应。
主动口服免疫对BALB/c和CBA/CaH小鼠具有保护作用，可减少再次感染后的真菌负荷和感染持续时间，而全身免疫未能预防随后的口腔感染。初次和二次口服感染后，血清中检测到的主要抗体是念珠菌特异性IgM；然而，未检测到念珠菌特异性唾液IgA。通过淋巴细胞或免疫血清的被动转移进行免疫，对易感或抗性小鼠品系的口腔感染均未提供任何显著的保护作用。
数据表明，口服途径主动免疫后，黏膜相关免疫可能发挥作用，最有可能是由口腔黏膜中的局部T淋巴细胞发挥作用，但没有证据支持体液免疫在预防口腔念珠菌病中发挥作用。
