Nishida Noriuki, Katamine Shigeru, Manuelidis Laura
Yale Medical School, New Haven, CT 06510, USA.
Science. 2005 Oct 21;310(5747):493-6. doi: 10.1126/science.1118155.
Infection of mice with an attenuated Creutzfeldt-Jakob disease agent (SY-CJD) interferes with superinfection by a more virulent human-derived CJD agent (FU-CJD) and does not require pathological prion protein (PrPres). Using a rapid coculture system, we found that a neural cell line free of immune system cells similarly supported substantial CJD agent interference without PrPres. In addition, SY-CJD prevented superinfection by sheep-derived Chandler (Ch) and 22L scrapie agents. However, only 22L and not Ch prevented FU-CJD infection, even though both scrapie strains provoked abundant PrPres. This relationship between particular strains of sheep- and human-derived agents is likely to affect their prevalence and epidemic spread.
用减毒的克雅氏病病原体（SY-CJD）感染小鼠会干扰毒性更强的人源克雅氏病病原体（FU-CJD）的重复感染，且不需要病理性朊病毒蛋白（PrPres）。使用快速共培养系统，我们发现一个不含免疫系统细胞的神经细胞系同样能在没有PrPres的情况下支持显著的克雅氏病病原体干扰。此外，SY-CJD可预防绵羊源钱德勒（Ch）毒株和22L痒病病原体的重复感染。然而，尽管两种痒病毒株都能引发大量PrPres，但只有22L毒株而非Ch毒株能预防FU-CJD感染。绵羊源和人源病原体的特定毒株之间的这种关系可能会影响它们的流行程度和传播范围。
