López-Boado Yolanda S, Cobb Laura M, Deora Rajendar
Section on Molecular Medicine, Department of Internal Medicine, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA.
Infect Immun. 2005 Nov;73(11):7525-34. doi: 10.1128/IAI.73.11.7525-7534.2005.
Motility is an important virulence phenotype for many bacteria, and flagellin, the monomeric component of flagella, is a potent proinflammatory factor. Of the three Bordetella species, Bordetella pertussis and Bordetella parapertussis are nonmotile human pathogens, while Bordetella bronchiseptica expresses flagellin and causes disease in animals and immunocompromised human hosts. The BvgAS two-component signal transduction system regulates phenotypic-phase transition (Bvg+, Bvg-, and Bvg(i)) in bordetellae. The Bvg- phase of B. bronchiseptica is characterized by the expression of flagellin and the repression of adhesins and toxins necessary for the colonization of the respiratory tract. B. bronchiseptica naturally infects a variety of animal hosts and constitutes an excellent model to study Bordetella pathogenesis. Using in vitro coculture models of bacteria and human lung epithelial cells, we studied the effects of B. bronchiseptica flagellin on host defense responses. Our results show that B. bronchiseptica flagellin is a potent proinflammatory factor that induces chemokine, cytokine, and host defense gene expression. Furthermore, we investigated receptor specificity in the response to B. bronchiseptica flagellin. Our results show that B. bronchiseptica flagellin is able to signal effectively through both human and mouse Toll-like receptor 5.
运动性是许多细菌的一种重要毒力表型，鞭毛蛋白作为鞭毛的单体成分，是一种强效促炎因子。在博德特氏菌属的三个菌种中，百日咳博德特氏菌和副百日咳博德特氏菌是不具运动性的人类病原体，而支气管败血博德特氏菌表达鞭毛蛋白，并在动物和免疫功能低下的人类宿主中引发疾病。BvgAS双组分信号转导系统调节博德特氏菌的表型相变（Bvg+、Bvg-和Bvg(i)）。支气管败血博德特氏菌的Bvg-相的特征是鞭毛蛋白的表达以及呼吸道定植所需的粘附素和毒素的抑制。支气管败血博德特氏菌自然感染多种动物宿主，是研究博德特氏菌发病机制的理想模型。利用细菌与人类肺上皮细胞的体外共培养模型，我们研究了支气管败血博德特氏菌鞭毛蛋白对宿主防御反应的影响。我们的结果表明，支气管败血博德特氏菌鞭毛蛋白是一种强效促炎因子，可诱导趋化因子、细胞因子和宿主防御基因表达。此外，我们研究了对支气管败血博德特氏菌鞭毛蛋白反应中的受体特异性。我们的结果表明，支气管败血博德特氏菌鞭毛蛋白能够通过人类和小鼠的Toll样受体5有效发出信号。
