Reich Reuven, Vintman Lina, Nielsen Søren, Kaern Janne, Bedrossian Carlos, Berner Aasmund, Davidson Ben
Department of Pharmacology and Experimental Therapeutics, Faculty of Medicine, Hebrew University, Jerusalem, Israel.
Diagn Cytopathol. 2005 Nov;33(5):332-7. doi: 10.1002/dc.20296.
Expression of the 67-kd laminin receptor (67-kd LR) has been reported in a wide range of carcinomas, in many of which it correlated with poor differentiation, metastasis, disease progression, and poor survival. Malignant mesothelioma (MM) is a locally aggressive and highly lethal tumor of serosal cavities that is rarely associated with clinically detectable metastasis to distant organs. The aim of this study was to analyze the expression of the 67-kd LR in epithelioid MM and carcinomas in effusions. Effusions from patients diagnosed with ovarian (=24) and breast (=38) adenocarcinomas and MM (=24) (total = 86) were analyzed for 67-kd LR protein expression, using immunocytochemistry. The 67-kd LR mRNA expression was additionally analyzed in 21 MM effusions using reverse transcription-polymerase chain reaction (RT-PCR). Protein expression of the 67-kd LR was frequently detected in carcinomas (19/24 ovarian tumors, 79%; 15/38 breast tumors, 39%), but was rare in MM (2/24 cases, 8%), despite the presence of mRNA transcripts for the receptor in all 21 specimens studied using RT-PCR. Nine benign effusions that were additionally studied for protein expression were uniformly negative, as were all reactive mesothelial cells in malignant effusions. Our results suggest that the 67-kd LR may aid in the differential diagnosis between metastatic carcinoma, mainly of ovarian origin, and MM. They additionally suggest that the failure of MM to express the 67-kd LR protein, as opposed to the frequent expression in carcinomas with proven metastatic capacity, may be one of the factors contributing to the reduced ability of the former tumor to metastasize to distant organs.
据报道，67-kd层粘连蛋白受体（67-kd LR）在多种癌症中均有表达，其中许多癌症与低分化、转移、疾病进展和不良预后相关。恶性间皮瘤（MM）是一种发生于浆膜腔的具有局部侵袭性且高度致命的肿瘤，很少发生临床可检测到的远处器官转移。本研究旨在分析67-kd LR在上皮样MM和积液性癌中的表达情况。采用免疫细胞化学方法，对86例患者（卵巢腺癌24例、乳腺癌38例和MM 24例）的积液进行67-kd LR蛋白表达分析。另外，采用逆转录-聚合酶链反应（RT-PCR）对21例MM积液进行67-kd LR mRNA表达分析。在癌组织中经常检测到67-kd LR蛋白表达（19/24例卵巢肿瘤，79%；15/38例乳腺肿瘤，39%），但在MM中很少见（2/24例，8%），尽管在所有21份采用RT-PCR研究的标本中均存在该受体的mRNA转录本。另外研究的9例良性积液的蛋白表达均为阴性，恶性积液中的所有反应性间皮细胞也均为阴性。我们的结果表明，67-kd LR可能有助于主要源于卵巢的转移性癌与MM之间的鉴别诊断。结果还表明，与具有已证实转移能力的癌组织中频繁表达相反，MM未能表达67-kd LR蛋白可能是导致前一种肿瘤向远处器官转移能力降低的因素之一。
