Satrutdinov A D, Chistiakova T I, Dediukhina E G, Kaparullina E N, Eroshin V K
Prikl Biokhim Mikrobiol. 2005 Sep-Oct;41(5):535-40.
A novel strain of bacteria (LPM-4) was isolated that is characterized by a unique EDTA requirement for cell growth. Suspensions of washed cells of strain LPM-4 degrated EDTA complexes with Ba2+, Mg 2+, Ca2+, and Mn2+ at constant rates (0.310-0.486 mmol EDTA/(g h)) and Zn-EDTA at an initial rate of 0.137 +/- 0.016 mmol EDTA/(g h). The temperature optima for cell growth and EDTA degradation were determined under pH-auxostat cultivation. As compared with the known EDTA-degrating bacteria, strain LPM-4 exhibited a higher specific growth rate (0.095 h(-1)) and lower mass cell yield (0.219 g cells/g EDTA) that is promising for its practical applications for EDTA removal in wastewater treatment plants.
分离出一种新型细菌菌株（LPM - 4），其特征是细胞生长对EDTA有独特需求。LPM - 4菌株洗涤细胞的悬浮液以恒定速率（0.310 - 0.486 mmol EDTA/（g·h））降解与Ba2 +、Mg2 +、Ca2 +和Mn2 +形成的EDTA络合物，降解Zn - EDTA的初始速率为0.137±0.016 mmol EDTA/（g·h）。在pH - 恒化器培养条件下确定了细胞生长和EDTA降解的最适温度。与已知的EDTA降解细菌相比，LPM - 4菌株表现出更高的比生长速率（0.095 h-1）和更低的细胞质量产率（0.219 g细胞/g EDTA），这使其在废水处理厂去除EDTA的实际应用中具有前景。
