Kaparullina Elena, Doronina Nina, Chistyakova Tatyana, Trotsenko Yuri
Skryabin Institute of Biochemistry and Physiology of Microorganisms, RAS and Pushchino State University, Pushchino, Moscow Region 142290, Russia.
Syst Appl Microbiol. 2009 May;32(3):157-62. doi: 10.1016/j.syapm.2008.12.003. Epub 2009 Feb 11.
A new ethylenediaminetetraacetic acid (EDTA)-utilizing gammaproteobacterial strain LPM-5(T) was isolated from municipal sewage sludge. Aerobic, gram-negative, motile rods multiply by binary fission. Neutrophilic and mesophilic, these are unable to grow in the presence of 3% NaCl (w/v), and unable to reduce nitrate to nitrite, and are oxidase and catalase positive, but lipase negative. The major cellular fatty acids are C(i15:0), C(a15:0) and C(16:1w7c). The dominant phospholipids are phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol (cardiolipin). The DNA G+C content is 68.3mol% (T(m)). The 16S rRNA gene sequence analysis showed a high similarity of strain LPM-5(T) to the species members of genus Stenotrophomonas: S. maltophilia LMG 958(T) (98.6%), S. rhizophila CCUG 47042(T) (98.3%), S. koreensis TR6-01(T) (97.6%) and S. acidaminiphila CIP 106456(T) (97.0%). Based on these results and modest DNA-DNA hybridization levels with S. maltophilia VKM B-591(T) (=LMG 958(T)) (51%) and S. rhizophila CCUG 47042(T) (52%), the isolate was classified as a novel species, Stenotrophomonas chelatiphaga sp. nov. (type strain LPM-5(T)=VKM B-2486=DSM-21508=CCUG 57178).
从城市污水污泥中分离出一株利用乙二胺四乙酸（EDTA）的新型γ-变形菌LPM-5(T)。该菌为需氧、革兰氏阴性、具运动性的杆菌，通过二分裂繁殖。嗜中性且嗜温，在3% NaCl（w/v）存在时无法生长，不能将硝酸盐还原为亚硝酸盐，氧化酶和过氧化氢酶呈阳性，但脂肪酶呈阴性。主要细胞脂肪酸为C(i15:0)、C(a15:0)和C(16:1w7c)。主要磷脂为磷脂酰乙醇胺、磷脂酰甘油和双磷脂酰甘油（心磷脂）。DNA G+C含量为68.3mol%（Tm）。16S rRNA基因序列分析表明，菌株LPM-5(T)与嗜麦芽窄食单胞菌属的物种成员具有高度相似性：嗜麦芽窄食单胞菌LMG 958(T)（98.6%）、嗜根窄食单胞菌CCUG 47042(T)（98.3%）、韩国窄食单胞菌TR6-01(T)（97.6%）和嗜酸窄食单胞菌CIP 106456(T)（97.0%）。基于这些结果以及与嗜麦芽窄食单胞菌VKM B-591(T)（=LMG 958(T)）（51%）和嗜根窄食单胞菌CCUG 47042(T)（52%）适度的DNA-DNA杂交水平，该分离株被分类为一个新物种，嗜螯窄食单胞菌（Stenotrophomonas chelatiphaga sp. nov.）（模式菌株LPM-5(T)=VKM B-2486=DSM-21508=CCUG 57178）。
