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[地衣芽孢杆菌的蛋白水解酶和溶杆菌属XL1的溶菌酰胺酶对普通变形杆菌和奇异变形杆菌的作用]

[Effect of the proteolytic enzymes of Bacillus licheniformis and the lysoamidase of Lysobacter sp. XL1 on Proteus vulgaris and Proteus mirabilis].

作者信息

Riazanova L P, Ledova L A, Tsurikova N V, Stepnaia O A, Sinitsyn A P, Kulaev I S

出版信息

Prikl Biokhim Mikrobiol. 2005 Sep-Oct;41(5):558-63.

Abstract

Preparations of culture liquid of three Bacullus licheniformis strains (S, 103, and 60.4) and the enzymatic preparation lysoamidase from culture liquid of Lysobacter sp. strain XL1 actively lysed preliminarily autoclaved cells of gram-negative bacteria Proteus vulgaris and P. mirabilis. Living Proteus cells treated with these enzymatic preparations were lysed during their subsequent autoclaving. Inoculation of enzyme-treated Proteus cells, taken either separately or in combination with one another and polymyxin B, into a rich medium led to cell repair and restoration of viability of culture.

摘要

三种地衣芽孢杆菌菌株(S、103和60.4)的培养液制剂,以及来自溶杆菌属菌株XL1培养液的酶制剂溶菌淀粉酶,能有效裂解经初步高压灭菌的革兰氏阴性菌普通变形杆菌和奇异变形杆菌的细胞。用这些酶制剂处理过的活变形杆菌细胞在随后的高压灭菌过程中会被裂解。将经酶处理的变形杆菌细胞单独或相互组合并与多粘菌素B一起接种到丰富培养基中,会导致细胞修复并恢复培养物的活力。

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