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对硝基苯基甘油对变形杆菌群集运动的抑制作用:一般特性

Abolition of swarming of Proteus by p-nitrophenyl glycerin: general properties.

作者信息

Williams F D

出版信息

Appl Microbiol. 1973 May;25(5):745-50. doi: 10.1128/am.25.5.745-750.1973.

DOI:10.1128/am.25.5.745-750.1973
PMID:4577177
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC380905/
Abstract

Para-nitrophenyl glycerin (PNPG) was shown to be an effective agent to abolish the swarming of Proteus mirabilis and Proteus vulgaris on predried solid culture media. The level required to abolish swarming varied with the strain of Proteus, the components of the medium, and also with the conditions of incubation. Generally 0.1 to 0.2 mM PNPG effectively abolished swarming for at least 24 h with aerobic incubation. Levels of PNPG that abolished swarming showed no effect upon the growth of the cells, little or no effect upon the motility characteristics of the organisms, and no effect upon the cellular morphology. PNPG was found to be freely water soluble, stable to autoclaving, and to retain biological activity for at least one month in prepared culture media stored under refrigeration.

摘要

对硝基苯基甘油(PNPG)被证明是一种有效的试剂,可消除奇异变形杆菌和普通变形杆菌在预干燥固体培养基上的群游现象。消除群游所需的浓度因变形杆菌菌株、培养基成分以及培养条件而异。一般来说,0.1至0.2 mM的PNPG在需氧培养条件下可有效消除群游现象至少24小时。能消除群游的PNPG浓度对细胞生长没有影响,对生物体的运动特性影响很小或没有影响,对细胞形态也没有影响。发现PNPG可自由溶于水,对高压灭菌稳定,并且在冷藏保存的制备培养基中至少一个月保持生物活性。

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8
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本文引用的文献

1
The Inhibition of the Spreading Growth of Proteus and other Bacteria to Permit the Isolation of Associated Streptococci.抑制变形杆菌及其他细菌的扩散生长以利于相关链球菌的分离
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Inhibition of Swarming of Proteus by Sodium Tetradecyl Sulfate, beta-Phenethyl Alcohol, and p-Nitrophenylglycerine.十四烷基硫酸钠、β-苯乙醇和对硝基苯基甘油对变形杆菌群集运动的抑制作用
Appl Microbiol. 1966 Nov;14(6):873-8. doi: 10.1128/am.14.6.873-878.1966.
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CELLULAR CHANGES ACCOMPANYING THE SWARMING OF PROTEUS MIRABILIS. I. OBSERVATIONS OF LIVING CULTURES.奇异变形杆菌群集相关的细胞变化。I. 活培养物观察
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TRANSDUCTION OF SWARMING IN PROTEUS MIRABILIS.奇异变形杆菌群体游动的转导
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The influence of medium composition on the growth and swarming of Proteus.培养基成分对变形杆菌生长和群集运动的影响。
J Gen Microbiol. 1967 Jun;47(3):369-78. doi: 10.1099/00221287-47-3-369.
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The swarming of proteus on semisynthetic media.
J Med Microbiol. 1971 Feb;4(1):1-11. doi: 10.1099/00222615-4-1-1.
7
Abolition of swarming of Proteus by p-nitrophenyl glycerin: application to blood agar media.对硝基苯基甘油抑制变形杆菌群集现象:在血琼脂培养基中的应用
Appl Microbiol. 1973 May;25(5):751-4. doi: 10.1128/am.25.5.751-754.1973.