Båth Klara, Roos Stefan, Wall Torun, Jonsson Hans
Department of Microbiology, Swedish University of Agricultural Sciences, Box 7025, SE-750 07 Uppsala, Sweden.
FEMS Microbiol Lett. 2005 Dec 1;253(1):75-82. doi: 10.1016/j.femsle.2005.09.042. Epub 2005 Oct 10.
Bioinformatical analyses of a draft genome sequence of the commensal bacterium Lactobacillus reuteri ATCC 55730 revealed 126 genes encoding putative extracellular proteins. The function, localization and distribution in bacterial species were predicted. Interestingly, few proteins possessed LPXTG motifs or C-terminal transmembrane anchors. Instead eight proteins were putatively anchored by GW repeats and several secreted proteins were likely to be re-associated to the surface. The majority of the extracellular proteins were widely distributed, i.e., found universally or in gram-positive bacteria, but 24 were only detected in L. reuteri. Further, the number of transporters was lower, while the number of enzyme was higher than in related species.
对共生细菌罗伊氏乳杆菌ATCC 55730的基因组序列草图进行的生物信息学分析显示，有126个基因编码假定的细胞外蛋白。预测了这些蛋白在细菌物种中的功能、定位和分布。有趣的是，很少有蛋白具有LPXTG基序或C端跨膜锚定结构。相反，有8种蛋白可能通过GW重复序列锚定，并且几种分泌蛋白可能重新与表面结合。大多数细胞外蛋白分布广泛，即在所有细菌或革兰氏阳性细菌中都能找到，但有24种仅在罗伊氏乳杆菌中检测到。此外，与相关物种相比，转运蛋白的数量较少，而酶的数量较多。
