Rakici Ozlem, Kiziltepe Ugursay, Coskun Bahar, Aslamaci Sait, Akar Fatma
Department of Pharmacology, Faculty of Pharmacy, Gazi University, Ankara, Turkey.
Int J Cardiol. 2005 Nov 2;105(2):209-15. doi: 10.1016/j.ijcard.2005.01.013.
The polyphenolic compound resveratrol presented in red wine has potent cardiovascular effect in animal. Here, we investigated the ability of resveratrol to relax human coronary bypass grafts, saphenous vein and internal mammary artery and also its effect on their endothelial reactivity.
Vascular rings were obtained from 38 male patients undergoing coronary artery bypass operation. The relaxant effects of resveratrol (10-70 microM) and acetylcholine (10(-8)-10(-4) M) were examined on precontracted saphenous vein and internal mammary artery rings.
Resveratrol, at concentration of 70 microM caused relaxations of 34.2+/-5.7% in saphenous vein and 35.2+/-5.4% in internal mammary artery. Endothelium removal and l-NOARG (nitric oxide synthase inhibitor, 10(-4) M) pretreatment almost completely inhibited the relaxation to resveratrol in internal mammary artery but partially in saphenous vein rings. Indomethacin (cyclooxygenase inhibitor, 10(-5) M) slightly, but not significantly enhanced the relaxation to resveratrol in both vessels. The endothelium-dependent relaxations to acetylcholine were significantly improved in the presence of resveratrol of 20 microM in both grafts (E(max): 33.8+/-3.7% versus 46.8+/-4% in saphenous vein n=9; p<0.05; 54. 4+/-5.3% versus 69.3+/-5.4% in internal mammary artery, n=8, p<0.05). The relaxations to acetylcholine were fully eliminated by combination of resveratrol with l-NOARG (10(-4) M) in both vessels.
Resveratrol produced mainly endothelium-dependent and nitric oxide-mediated vasodilation in human internal mammary artery but partially in saphenous vein rings and improved their endothelial reactivity. This may have a therapeutic potential in cardiovascular diseases.
红酒中含有的多酚化合物白藜芦醇在动物实验中具有强大的心血管效应。在此，我们研究了白藜芦醇舒张人冠状动脉搭桥移植物、大隐静脉和乳内动脉的能力及其对这些血管内皮反应性的影响。
从38例接受冠状动脉搭桥手术的男性患者获取血管环。检测白藜芦醇（10 - 70微摩尔）和乙酰胆碱（10⁻⁸ - 10⁻⁴摩尔）对预先收缩的大隐静脉和乳内动脉血管环的舒张作用。
浓度为70微摩尔的白藜芦醇使大隐静脉舒张34.2±5.7%，使乳内动脉舒张35.2±5.4%。去除内皮和用L - NOARG（一氧化氮合酶抑制剂，10⁻⁴摩尔）预处理几乎完全抑制了乳内动脉对白藜芦醇的舒张反应，但仅部分抑制了大隐静脉血管环的反应。吲哚美辛（环氧化酶抑制剂，10⁻⁵摩尔）轻微但不显著地增强了两种血管对白藜芦醇的舒张反应。在两种移植物中，20微摩尔白藜芦醇存在时，对乙酰胆碱的内皮依赖性舒张反应显著改善（E(max)：大隐静脉中为33.8±3.7%对46.8±4%，n = 9；p < 0.05；乳内动脉中为54.4±5.3%对69.3±5.4%，n = 8，p < 0.05）。白藜芦醇与L - NOARG（10⁻⁴摩尔）联合使用完全消除了两种血管对乙酰胆碱的舒张反应。
白藜芦醇在人乳内动脉中主要产生内皮依赖性且由一氧化氮介导的血管舒张，在大隐静脉血管环中部分如此，并改善了它们的内皮反应性。这可能在心血管疾病治疗中具有潜在应用价值。
