Pörtner Ralf, Nagel-Heyer Stephanie, Goepfert Christiane, Adamietz Peter, Meenen Norbert M
Technische Universität Hamburg-Harburg, Bioprozess- und Bioverfahrenstechnik, Denickestr. 15, 21071 Hamburg, Germany.
J Biosci Bioeng. 2005 Sep;100(3):235-45. doi: 10.1263/jbb.100.235.
Bioreactor systems play an important role in tissue engineering, as they enable reproducible and controlled changes in specific environmental factors. They can provide technical means to perform controlled studies aimed at understanding specific biological, chemical or physical effects. Furthermore, bioreactors allow for a safe and reproducible production of tissue constructs. For later clinical applications, the bioreactor system should be an advantageous method in terms of low contamination risk, ease of handling and scalability. To date the goals and expectations of bioreactor development have been fulfilled only to some extent, as bioreactor design in tissue engineering is very complex and still at an early stage of development. In this review we summarize important aspects for bioreactor design and provide an overview on existing concepts. The generation of three dimensional cartilage-carrier constructs is described to demonstrate how the properties of engineered tissues can be improved significantly by combining biological and engineering knowledge. In the future, a very intimate collaboration between engineers and biologists will lead to an increased fundamental understanding of complex issues that can have an impact on tissue formation in bioreactors.
生物反应器系统在组织工程中发挥着重要作用，因为它们能够在特定环境因素中实现可重复且可控的变化。它们可以提供技术手段来进行旨在了解特定生物、化学或物理效应的对照研究。此外，生物反应器能够安全且可重复地生产组织构建体。对于后续的临床应用而言，生物反应器系统在低污染风险、易于操作和可扩展性方面应是一种具有优势的方法。迄今为止，生物反应器开发的目标和期望仅在一定程度上得以实现，因为组织工程中的生物反应器设计非常复杂且仍处于早期开发阶段。在本综述中，我们总结了生物反应器设计的重要方面，并概述了现有概念。描述了三维软骨 - 载体构建体的生成，以展示如何通过结合生物学和工程学知识来显著改善工程组织的特性。未来，工程师和生物学家之间非常紧密的合作将使人们对可能影响生物反应器中组织形成的复杂问题有更深入的基本理解。
