Ito Takeshi, Nakashimada Yutaka, Senba Koichiro, Matsui Tomoaki, Nishio Naomichi
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Japan.
J Biosci Bioeng. 2005 Sep;100(3):260-5. doi: 10.1263/jbb.100.260.
H2 and ethanol production from glycerol-containing wastes discharged after a manufacturing process for biodiesel fuel (biodiesel wastes) using Enterobacter aerogenes HU-101 was evaluated. The biodiesel wastes should be diluted with a synthetic medium to increase the rate of glycerol utilization and the addition of yeast extract and tryptone to the synthetic medium accelerated the production of H2 and ethanol. The yields of H2 and ethanol decreased with an increase in the concentrations of biodiesel wastes and commercially available glycerol (pure glycerol). Furthermore, the rates of H2 and ethanol production from biodiesel wastes were much lower than those at the same concentration of pure glycerol, partially due to a high salt content in the wastes. In continuous culture with a packed-bed reactor using self-immobilized cells, the maximum rate of H2 production from pure glycerol was 80 mmol/l/h yielding ethanol at 0.8 mol/mol-glycerol, while that from biodiesel wastes was only 30 mmol/l/h. However, using porous ceramics as a support material to fix cells in the reactor, the maximum H2 production rate from biodiesel wastes reached 63 mmol/l/h obtaining an ethanol yield of 0.85 mol/mol-glycerol.
对利用产气肠杆菌HU-101从生物柴油燃料制造过程中排放的含甘油废物（生物柴油废物）生产氢气和乙醇进行了评估。生物柴油废物应与合成培养基稀释以提高甘油利用率，并且向合成培养基中添加酵母提取物和胰蛋白胨可加速氢气和乙醇的产生。氢气和乙醇的产量随着生物柴油废物和市售甘油（纯甘油）浓度的增加而降低。此外，生物柴油废物产生氢气和乙醇的速率远低于相同浓度纯甘油时的速率，部分原因是废物中盐含量高。在使用自固定化细胞的填充床反应器进行连续培养时，纯甘油产生氢气的最大速率为80 mmol/l/h，乙醇产率为0.8 mol/mol-甘油，而生物柴油废物产生氢气的最大速率仅为30 mmol/l/h。然而，使用多孔陶瓷作为载体材料在反应器中固定细胞，生物柴油废物产生氢气的最大速率达到63 mmol/l/h，乙醇产率为0.85 mol/mol-甘油。
