嗜热栖热菌ATCC35047的 kojibiose磷酸化酶基因和海藻糖磷酸化酶基因在枯草芽孢杆菌中的过表达以及利用两种磷酸化酶合成selaginose

Hyper expression of kojibiose phosphorylase gene and trehalose phosphorylase gene from Thermoanaerobacter brockii ATCC35047 in Bacillus subtilis and selaginose synthesis utilizing two phosphorylases.

作者信息

Yamamoto Takuo, Mukai Kazuhisa, Maruta Kazuhiko, Watanabe Hikaru, Yamashita Hiroshi, Nishimoto Tomoyuki, Kubota Michio, Chaen Hiroto, Fukuda Shigeharu

机构信息

Amase Institute, Hayashibara Biochemical Laboratories, Inc., 7-7 Amase Minami-machi, Okayama 700-0834, Japan.

出版信息

J Biosci Bioeng. 2005 Sep;100(3):343-6. doi: 10.1263/jbb.100.343.

DOI:10.1263/jbb.100.343

PMID:16243288

Abstract

The kojibiose phosphorylase (KP) gene and trehalose phosphorylase (TP) gene from Thermoanaerobacter brockii ATCC35047 were intracellularly hyper-expressed under the control of the Bacillus amyloliquefaciens alpha-amylase promoter in Bacillus subtilis. The production yields were estimated to be 2.1 g of KP and 4.9 g of TP per liter of medium. Selaginose, non-reducing trisaccharide, was synthesized from trehalose utilizing the recombinant KP and TP from B. subtilis. Selaginose was not hydrolyzed by salivary amylase, artificial gastric juice, pancreatic amylase, or small intestinal enzymes.

摘要

来自布氏嗜热厌氧菌ATCC35047的 kojibiose磷酸化酶（KP）基因和海藻糖磷酸化酶（TP）基因在枯草芽孢杆菌中受解淀粉芽孢杆菌α-淀粉酶启动子控制进行细胞内超表达。估计每升培养基的产量为2.1克KP和4.9克TP。利用枯草芽孢杆菌的重组KP和TP从海藻糖合成了非还原性三糖selaginose。Selaginose不会被唾液淀粉酶、人工胃液、胰淀粉酶或小肠酶水解。