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海马齿叶精油的化学成分及生物活性

Chemical composition and biological activities of essential oil from the leaves of Sesuvium portulacastrum.

作者信息

Magwa Michael L, Gundidza Mazuru, Gweru Nyasha, Humphrey Godfred

机构信息

Department of Botany and Electron Microscope Unit, University of Fort Hare, Eastern Cape, South Africa.

出版信息

J Ethnopharmacol. 2006 Jan 3;103(1):85-9. doi: 10.1016/j.jep.2005.07.024. Epub 2005 Oct 21.

Abstract

Sesuvium portulacastrum has long been used as a remedy for fever and scurvy. Hydrodistillation was used to extract the essential oil from the fresh leaves of Sesuvium portulacastrum. The essential oil yield obtained was 0.15%. Using GC-MS analysis, alpha-pinene, camphene, beta-pinene, alpha-terpinene, O-cymene, limonene, 1,8-cineole, alpha-terpinene, bornyl acetate, tridecane, trans-caryophyllene and alpha-humulene were identified. The hole plate diffusion method was used for antibacterial testing. The essential oil exhibited antibacterial activity against Acetobacter calcoacetica, Bacillus subtillis, Clostridium sporogenes, Clostridium perfringens, Escherichia coli, Salmonella typhii, Staphylococcus aureus and Yersinia enterocolitica. The mycelium growth inhibition method was used for the antifungal testing. The oil exhibited antifungal activity against Candida albicans, Aspergillus niger, Aspergillus flavus and Penicillium notatum. Using the beta-carotene, acetone and linoleic acid method for the antioxidant testing, the essential oil showed antioxidant activity threshold of 15.9 mm mean zone of color retention.

摘要

海马齿长期以来被用作治疗发烧和坏血病的药物。采用水蒸馏法从海马齿新鲜叶片中提取精油。所获得的精油产率为0.15%。通过气相色谱-质谱联用(GC-MS)分析,鉴定出了α-蒎烯、莰烯、β-蒎烯、α-松油烯、邻伞花烃、柠檬烯、1,8-桉叶素、α-松油烯、乙酸龙脑酯、十三烷、反式石竹烯和α-葎草烯。采用打孔平板扩散法进行抗菌测试。该精油对醋酸钙不动杆菌、枯草芽孢杆菌、产芽孢梭菌、产气荚膜梭菌、大肠杆菌、伤寒沙门氏菌、金黄色葡萄球菌和小肠结肠炎耶尔森氏菌表现出抗菌活性。采用菌丝体生长抑制法进行抗真菌测试。该精油对白色念珠菌、黑曲霉、黄曲霉和青霉表现出抗真菌活性。使用β-胡萝卜素、丙酮和亚油酸法进行抗氧化测试,该精油的抗氧化活性阈值为平均颜色保留区15.9毫米。

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